ausblenden:
Schlagwörter:
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Zusammenfassung:
The CRISPR technology has revolutionized reverse genetics and has a major impact on biomedical research. In C. elegans, various CRISPR-associated approaches have been established that have further increased the methodological toolkit of this organism. In the applied sciences, plant and animal parasitic nematodes might profit substantially from the development of related tools. We study the free-living nematode Pristionchus pacificus and have intensive experience with transferring methodologies originally developed in C. elegans to P. pacificus. P. pacificus is a model system for integrative evolutionary biology with a sophisticated toolkit for functional analysis. It has a 4-day generation time (20° C) and is a self-fertilizing hermaphrodite similar to C. elegans. Recent studies in this species focus on the regulation of predatory vs bacterial feeing as a result of mouth-form developmental plasticity. In this talk, we will provide an overview on CRISPR applications in C. elegans and P. pacificus. In particular, we will discuss successful troubleshooting and potential modifications of standard protocols that might make it more amenable, also to parasitic nematode species.
