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  MINSTED fluorescence localization and nanoscopy

Weber, M., Leutenegger, M., Stoldt, S., Jakobs, S., Mihaila, T. S., Butkevich, A., et al. (2021). MINSTED fluorescence localization and nanoscopy. Nature Photonics, 15, 361-366. doi:10.1038/s41566-021-00774-2.

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Item Permalink: https://hdl.handle.net/21.11116/0000-000F-05F3-9 Version Permalink: https://hdl.handle.net/21.11116/0000-000F-05F4-8
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 Creators:
Weber, Michael, Author
Leutenegger, Marcel, Author
Stoldt, Stefan, Author
Jakobs, Stefan, Author
Mihaila, Tiberiu S., Author
Butkevich, Alexey1, Author           
Hell, Stefan W.1, Author                 
Affiliations:
1Optical Nanoscopy, Max Planck Institute for Medical Research, Max Planck Society, ou_2364730              

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 Abstract: We introduce MINSTED, a fluorophore localization and super-resolution microscopy concept based on stimulated emission depletion (STED) that provides spatial precision and resolution down to the molecular scale. In MINSTED, the intensity minimum of the STED doughnut, and hence the point of minimal STED, serves as a movable reference coordinate for fluorophore localization. As the STED rate, the background and the required number of fluorescence detections are low compared with most other STED microscopy and localization methods, MINSTED entails substantially less fluorophore bleaching. In our implementation, 200–1,000 detections per fluorophore provide a localization precision of 1–3 nm in standard deviation, which in conjunction with independent single fluorophore switching translates to a ~100-fold improvement in far-field microscopy resolution over the diffraction limit. The performance of MINSTED nanoscopy is demonstrated by imaging the distribution of Mic60 proteins in the mitochondrial inner membrane of human cells.

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Language(s): eng - English
 Dates: 2020-10-302021-02-012021-03-152021
 Publication Status: Issued
 Pages: 9
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1038/s41566-021-00774-2
 Degree: -

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Title: Nature Photonics
Source Genre: Journal
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Publ. Info: London [u.a.] : Nature Publ. Group
Pages: - Volume / Issue: 15 Sequence Number: - Start / End Page: 361 - 366 Identifier: Other: 1749-4885
Other: 1749-4893
CoNE: https://pure.mpg.de/cone/journals/resource/1000000000240270