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  Enhanced assembly of bacteriophage T7 produced in cell-free reactions under simulated microgravity

Lehr, F.-X., Pavletić, B., Glatter, T., Heimerl, T., Moeller, R., & Niederholtmeyer, H. (2024). Enhanced assembly of bacteriophage T7 produced in cell-free reactions under simulated microgravity. npj Microgravity, 10: 30. doi:10.1038/s41526-024-00378-4.

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Item Permalink: https://hdl.handle.net/21.11116/0000-000F-1417-1 Version Permalink: https://hdl.handle.net/21.11116/0000-000F-2ABA-1
Genre: Journal Article

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https://doi.org/10.1038/s41526-024-00378-4 (Publisher version)
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OA-Status:
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 Creators:
Lehr, Francois-Xavier1, Author           
Pavletić, Bruno2, Author
Glatter, Timo3, Author                 
Heimerl, Thomas2, Author
Moeller, Ralf2, Author
Niederholtmeyer, Henrike1, 2, Author                 
Affiliations:
1Emmy Noether research Group Cell-free Synthetic Biology, Max Planck Institute for Terrestrial Microbiology, Max Planck Society, ou_3266293              
2external, ou_persistent22              
3Core Facility Mass Spectrometry and Proteomics, Max Planck Institute for Terrestrial Microbiology, Max Planck Society, ou_3266266              

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 Abstract: On-demand biomanufacturing has the potential to improve healthcare and self-sufficiency during space missions. Cell-free transcription and translation reactions combined with DNA blueprints can produce promising therapeutics like bacteriophages and virus-like particles. However, how space conditions affect the synthesis and self-assembly of such complex multi-protein structures is unknown. Here, we characterize the cell-free production of infectious bacteriophage T7 virions under simulated microgravity. Rotation in a 2D-clinostat increased the number of infectious particles compared to static controls. Quantitative analyses by mass spectrometry, immuno-dot-blot and real-time PCR showed no significant differences in protein and DNA contents, suggesting enhanced self-assembly of T7 phages in simulated microgravity. While the effects of genuine space conditions on the cell-free synthesis and assembly of bacteriophages remain to be investigated, our findings support the vision of a cell-free synthesis-enabled “astropharmacy”.

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Language(s): eng - English
 Dates: 2024-03-15
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: URI: https://doi.org/10.1038/s41526-024-00378-4
Other: Lehr2024
DOI: 10.1038/s41526-024-00378-4
 Degree: -

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Project name : -
Grant ID : NI 2040/1-1
Funding program : -
Funding organization : Deutsche Forschungsgemeinschaft (DFG)
Project name : ISS LIFE (Program RF-FuW, Teilprogramm: 475) GANDALF PhD Graduate School
Grant ID : -
Funding program : -
Funding organization : DLR (German Aerospace Center)
Project name : -
Grant ID : 4000132667/20/NL/CLR/pt
Funding program : -
Funding organization : ESA Topical Team Space Synthetic Biology
Project name : -
Grant ID : -
Funding program : Open Access
Funding organization : Project DEAL

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Title: npj Microgravity
Source Genre: Journal
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Publ. Info: -
Pages: 30 Volume / Issue: 10 Sequence Number: 30 Start / End Page: - Identifier: ISBN: 2373-8065