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  T4 phage RNA is NAD-capped and alters the NAD-cap epitranscriptome of Escherichia coli during infection through a phage-encoded decapping enzyme

Wolfram-Schauerte, M., Moskalchuk, A., Pozhydaieva, N., Ramírez Rojas, A. A., Schindler, D., Kaiser, S., et al. (2024). T4 phage RNA is NAD-capped and alters the NAD-cap epitranscriptome of Escherichia coli during infection through a phage-encoded decapping enzyme. bioRxiv: the preprint server for biology, 2024.04.04.588121.

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Wolfram-Schauerte, Maik1, Author           
Moskalchuk, Anastassiya1, Author
Pozhydaieva, Nadiia1, Author           
Ramírez Rojas, Adán Andrés2, Author           
Schindler, Daniel2, Author                 
Kaiser, Stefanie3, Author
Paczia, Nicole4, Author                 
Höfer, Katharina1, Author                 
Affiliations:
1Max Planck Research Group Bacterial Epitranscriptomics, Max Planck Institute for Terrestrial Microbiology, Max Planck Society, ou_3266299              
2Core Facility MPG MAXGenesys DNAfoundry, Max Planck Institute for Terrestrial Microbiology, Max Planck Society, ou_3266268              
3external, ou_persistent22              
4Core Facility Metabolomics and small Molecules Mass Spectrometry, Max Planck Institute for Terrestrial Microbiology, Max Planck Society, ou_3266267              

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 Abstract: Nicotinamide adenine dinucleotide (NAD) serves as a cap-like structure on cellular RNAs (NAD-RNAs) in all domains of life including the bacterium Escherichia coli. NAD also acts as a key molecule in phage-host interactions, where bacterial immune systems deplete NAD to abort phage infection. Nevertheless, NAD-RNAs have not yet been identified during phage infections of bacteria and the mechanisms of their synthesis and degradation are unknown in this context. The T4 phage that specifically infects E. coli presents an important model to study phage infections, but a systematic analysis of the presence and dynamics of NAD-RNAs during T4 phage infection is lacking. Here, we investigate the presence of NAD-RNAs during T4 phage infection in a dual manner. By applying time resolved NAD captureSeq, we identify NAD-capped host and phage transcripts and their dynamic regulation during phage infection. We provide evidence that NAD-RNAs are

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 Dates: 2024-04-04
 Publication Status: Issued
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 Rev. Type: No review
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Title: bioRxiv : the preprint server for biology
  Abbreviation : bioRxiv
Source Genre: Journal
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Pages: - Volume / Issue: - Sequence Number: 2024.04.04.588121 Start / End Page: - Identifier: ZDB: 2766415-6
CoNE: https://pure.mpg.de/cone/journals/resource/2766415-6