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  Genetic toolbox for Photorhabdus and Xenorhabdus: pSEVA based heterologous expression systems and CRISPR/Cpf1 based genome editing for rapid natural product profiling

Rill, A., Zhao, L., & Bode, H. B. (2024). Genetic toolbox for Photorhabdus and Xenorhabdus: pSEVA based heterologous expression systems and CRISPR/Cpf1 based genome editing for rapid natural product profiling. Microbial Cell Factories, 23: 98. doi:10.1186/s12934-024-02363-8.

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https://doi.org/10.1186/s12934-024-02363-8 (Verlagsversion)
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 Urheber:
Rill, Alexander1, Autor           
Zhao, Lei2, Autor
Bode, Helge B.1, 3, 4, 5, 6, Autor                 
Affiliations:
1Natural Product Function and Engineering, Department of Natural Products in Organismic Interactions, Max Planck Institute for Terrestrial Microbiology, Max Planck Society, ou_3266308              
2external, ou_persistent22              
3Chemical Biology, Department of Chemistry, Philipps University Marburg, Marburg, Germany, ou_persistent22              
4Molecular Biotechnology, Department of Biosciences, Goethe University Frankfurt, Frankfurt, Germany, External Organizations, ou_421891              
5Center for Synthetic Microbiology (SYNMIKRO), Philipps University Marburg, Germany, ou_persistent22              
6Senckenberg Gesellschaft für Naturforschung, Frankfurt, ou_persistent22              

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 Zusammenfassung: Bacteria of the genus Photorhabdus and Xenorhabdus are motile, Gram-negative bacteria that live in symbiosis with entomopathogenic nematodes. Due to their complex life cycle, they produce a large number of specialized metabolites (natural products) encoded in biosynthetic gene clusters (BGC). Genetic tools for Photorhabdus and Xenorhabdus have been rare and applicable to only a few strains. In the past, several tools have been developed for the activation of BGCs and the deletion of individual genes. However, these often have limited efficiency or are time consuming. Among the limitations, it is essential to have versatile expression systems and genome editing tools that could facilitate the practical work.

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 Datum: 2023-12-172024-03-112024-04-01
 Publikationsstatus: Erschienen
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: URI: https://doi.org/10.1186/s12934-024-02363-8
Anderer: Rill2024
DOI: 10.1186/s12934-024-02363-8
 Art des Abschluß: -

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Projektinformation

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Projektname : RhabdoFerm
Grant ID : -
Förderprogramm : -
Förderorganisation : BMBF
Projektname : SYNPEP
Grant ID : 835108
Förderprogramm : -
Förderorganisation : ERC advanced grant
Projektname : -
Grant ID : -
Förderprogramm : Open Access
Förderorganisation : Projekt DEAL

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Titel: Microbial Cell Factories
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: BioMed Central
Seiten: - Band / Heft: 23 Artikelnummer: 98 Start- / Endseite: - Identifikator: ISSN: 1475-2859
CoNE: https://pure.mpg.de/cone/journals/resource/111041294029026