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Abstract:
We will demonstrate the method for automated confocal imaging of a broad diversity of unicellular organisms ranging in size from 5 μm to 200μm. Cells are fixed and stained for visualising nuclei, membranes, chlorophyll and outer-surface by means of 3D-fluorescence microscopy. An adaptive feedback microscopy will be used, to set up a fully automated imaging pipeline with a high throughput. The workflow includes a low-resolution imaging of the entire preparation, the automated processing of these images for localising and selecting cells of interest, and subsequent automated triggering of slower 3D high-resolution acquisitions at relevant positions.
