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  Human surface ectoderm and amniotic ectoderm are sequentially specified according to cellular density

Nakanoh, S., Sham, K., Ghimire, S., Mohorianu, I., Rayon, T., & Vallier, L. (2024). Human surface ectoderm and amniotic ectoderm are sequentially specified according to cellular density. Science Advances, 10(9): eadh7748. doi:10.1126/sciadv.adh7748.

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© 2024 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science
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Nakanoh, Shota , Author
Sham, Kendig , Author
Ghimire, Sabitri, Author
Mohorianu, Irina , Author
Rayon, Teresa , Author
Vallier, Ludovic1, Author                 
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1Liver Organogenesis (Ludovic Vallier), Max Planck Fellow Group, Max Planck Institute for Molecular Genetics, Max Planck Society, ou_3486364              

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 Abstract: Mechanisms specifying amniotic ectoderm and surface ectoderm are unresolved in humans due to their close similarities in expression patterns and signal requirements. This lack of knowledge hinders the development of protocols to accurately model human embryogenesis. Here, we developed a human pluripotent stem cell model to investigate the divergence between amniotic and surface ectoderms. In the established culture system, cells differentiated into functional amnioblast-like cells. Single-cell RNA sequencing analyses of amnioblast differentiation revealed an intermediate cell state with enhanced surface ectoderm gene expression. Furthermore, when the differentiation started at the confluent condition, cells retained the expression profile of surface ectoderm. Collectively, we propose that human amniotic ectoderm and surface ectoderm are specified along a common nonneural ectoderm trajectory based on cell density. Our culture system also generated extraembryonic mesoderm-like cells from the primed pluripotent state. Together, this study provides an integrative understanding of the human nonneural ectoderm development and a model for embryonic and extraembryonic human development around gastrulation.

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Language(s): eng - English
 Dates: 2024-01-292024-03-01
 Publication Status: Published online
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 Rev. Type: Peer
 Identifiers: DOI: 10.1126/sciadv.adh7748
PMID: 38427729
PMC: PMC10906920
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Title: Science Advances
  Other : Sci. Adv.
Source Genre: Journal
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Publ. Info: Washington : AAAS
Pages: - Volume / Issue: 10 (9) Sequence Number: eadh7748 Start / End Page: - Identifier: ISSN: 2375-2548
CoNE: https://pure.mpg.de/cone/journals/resource/2375-2548