Deutsch
 
Hilfe Datenschutzhinweis Impressum
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT
  Human surface ectoderm and amniotic ectoderm are sequentially specified according to cellular density

Nakanoh, S., Sham, K., Ghimire, S., Mohorianu, I., Rayon, T., & Vallier, L. (2024). Human surface ectoderm and amniotic ectoderm are sequentially specified according to cellular density. Science Advances, 10(9): eadh7748. doi:10.1126/sciadv.adh7748.

Item is

Basisdaten

einblenden: ausblenden:
Genre: Zeitschriftenartikel

Dateien

einblenden: Dateien
ausblenden: Dateien
:
sciadv.adh7748.pdf (Verlagsversion), 12MB
Name:
sciadv.adh7748.pdf
Beschreibung:
-
OA-Status:
Keine Angabe
Sichtbarkeit:
Öffentlich
MIME-Typ / Prüfsumme:
application/pdf / [MD5]
Technische Metadaten:
Copyright Datum:
-
Copyright Info:
© 2024 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science
Lizenz:
-

Externe Referenzen

einblenden:

Urheber

einblenden:
ausblenden:
 Urheber:
Nakanoh, Shota , Autor
Sham, Kendig , Autor
Ghimire, Sabitri, Autor
Mohorianu, Irina , Autor
Rayon, Teresa , Autor
Vallier, Ludovic1, Autor                 
Affiliations:
1Liver Organogenesis (Ludovic Vallier), Max Planck Fellow Group, Max Planck Institute for Molecular Genetics, Max Planck Society, ou_3486364              

Inhalt

einblenden:
ausblenden:
Schlagwörter: -
 Zusammenfassung: Mechanisms specifying amniotic ectoderm and surface ectoderm are unresolved in humans due to their close similarities in expression patterns and signal requirements. This lack of knowledge hinders the development of protocols to accurately model human embryogenesis. Here, we developed a human pluripotent stem cell model to investigate the divergence between amniotic and surface ectoderms. In the established culture system, cells differentiated into functional amnioblast-like cells. Single-cell RNA sequencing analyses of amnioblast differentiation revealed an intermediate cell state with enhanced surface ectoderm gene expression. Furthermore, when the differentiation started at the confluent condition, cells retained the expression profile of surface ectoderm. Collectively, we propose that human amniotic ectoderm and surface ectoderm are specified along a common nonneural ectoderm trajectory based on cell density. Our culture system also generated extraembryonic mesoderm-like cells from the primed pluripotent state. Together, this study provides an integrative understanding of the human nonneural ectoderm development and a model for embryonic and extraembryonic human development around gastrulation.

Details

einblenden:
ausblenden:
Sprache(n): eng - English
 Datum: 2024-01-292024-03-01
 Publikationsstatus: Online veröffentlicht
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: DOI: 10.1126/sciadv.adh7748
PMID: 38427729
PMC: PMC10906920
 Art des Abschluß: -

Veranstaltung

einblenden:

Entscheidung

einblenden:

Projektinformation

einblenden:

Quelle 1

einblenden:
ausblenden:
Titel: Science Advances
  Andere : Sci. Adv.
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: Washington : AAAS
Seiten: - Band / Heft: 10 (9) Artikelnummer: eadh7748 Start- / Endseite: - Identifikator: ISSN: 2375-2548
CoNE: https://pure.mpg.de/cone/journals/resource/2375-2548