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  Atomic Force Microscopy Imaging and Force Spectroscopy of Supported Lipid Bilayers

Unsay, J. D., Cosentino, K., & García-Sáez, A. J. (2015). Atomic Force Microscopy Imaging and Force Spectroscopy of Supported Lipid Bilayers. Journal of Visualized Experiments, (101): e52867. doi:10.3791/52867.

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 Urheber:
Unsay, Joseph D.1, Autor
Cosentino, Katia1, Autor
García-Sáez, Ana J.2, 3, Autor                 
Affiliations:
1External Organizations, ou_persistent22              
2Interfaculty Institute of Biochemistry, Eberhard-Karls-Universität Tübingen, Tübingen, Germany, ou_persistent22              
3Max-Planck Institute for Intelligent Systems, Stuttgart, Germany, ou_persistent22              

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 Zusammenfassung: Atomic force microscopy (AFM) is a versatile, high-resolution imaging technique that allows visualization of biological membranes. It has sufficient magnification to examine membrane substructures and even individual molecules. AFM can act as a force probe to measure interactions and mechanical properties of membranes. Supported lipid bilayers are conventionally used as membrane models in AFM studies. In this protocol, we demonstrate how to prepare supported bilayers and characterize their structure and mechanical properties using AFM. These include bilayer thickness and breakthrough force. The information provided by AFM imaging and force spectroscopy help define mechanical and chemical properties of membranes. These properties play an important role in cellular processes such as maintaining cell hemostasis from environmental stress, bringing membrane proteins together, and stabilizing protein complexes.

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Sprache(n): eng - English
 Datum: 2015-07-22
 Publikationsstatus: Erschienen
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: DOI: 10.3791/52867
BibTex Citekey: unsay_atomic_2015
 Art des Abschluß: -

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Titel: Journal of Visualized Experiments
  Andere : Journal of Visualized Experiments: JoVE
  Kurztitel : J. Vis. Exp.
  Kurztitel : JoVE
Genre der Quelle: Zeitschrift
 Urheber:
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Ort, Verlag, Ausgabe: Rockville Pike, Bethesda MD : JoVE
Seiten: - Band / Heft: (101) Artikelnummer: e52867 Start- / Endseite: - Identifikator: ISSN: 1940-087X
CoNE: https://pure.mpg.de/cone/journals/resource/1940087X