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キーワード:
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要旨:
An optimized diet for in vitro rearing of honey bee (Apis mellifera Linnaeus 1758) larvae was developed. Based on lyophilized royal jelly, the diet comprises sugars, a fungicide and a vitamin formulation. All these components are available through biochemical supply companies. The protocol of the semiartificial diet is simple. Rate of growth and survival of bee larvae fed on the new diets were evaluated. In vitro development of worker larvae reared on the optimized diet was normal, resulting in a mortality of only about 10%.
