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We acknowledge T. White for assistance during the XFEL experiment and C. Uetrecht for valuable discussions. T.J.L. was supported by a Helmholtz Young Investigator Award. H.B., C.B., S.B., H.N.C., T.J.L. acknowledge financial support obtained from the Cluster of Excellence ‘Advanced Imaging of Matter’ of the Deutsche Forschungsgemeinschaft (DFG) - EXC 2056 - project ID 390715994. In addition, H.B. and C.B. received support from BMBF via projects 05K19GU4 and 05K20GUB. The Helmholtz association funded S.F., J.L. and A.M. through the project FISCOV and P.Y.A.R. and A.M. through FragX. The Helmholtz association further supported the project by financing P.Y.A.R, J.L. and A.M. via the Impulse and Networking funds InternLabs-0011 ‘HIR3X’. W.E. was supported through the BMBF-funded project “ConScience” (project 16GW0277) and the Röntgen-Angstrom cluster project “X-ray drug design platform” (13K22CHB). D.T. was supported by the Slovenian Research Agency (ARRS; research program P1-0048, Infrastructural program IO-0048). L.R. was financed by BMBF project 13K18FLA. We acknowledge European XFEL in Schenefeld, Germany, for provision of x-ray free-electron laser beamtime at SPB/SFX as part of proposal 2696 and would like to thank the staff for their assistance. The authors are indebted to the XFEL Biology Infrastructure (XBI) for the provision of instrumentation that has enabled this experiment. Sample reservoirs and the anti-settling device employed in parts of the measurements presented here were designed and fabricated by the Max Planck Institute for Medical Research, Heidelberg, which also provided instruction in its use. This research was supported through computational resources (Maxwell cluster) and experimental facilities (PETRA-III beamline P11) operated by Deutsches Elektronen-Synchrotron DESY, Hamburg, Germany, a member of the Helmholtz Association HGF. We acknowledge the P11 staff for their invaluable help. All authors are indebted to Galen Correy, as well as an anonymous reviewer, for constructive criticism during the review process. Open Access funding enabled and organized by Projekt DEAL.
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