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  Phosphorylation of Nup98 by multiple kinases is crucial for NPC disassembly during mitotic entry

Laurell, E., Beck, K., Krupina, K., Theerthagiri, G., Bodenmiller, B., Horvath, P., Aebersold, R., Antonin, W., & Kutay, U. (2011). Phosphorylation of Nup98 by multiple kinases is crucial for NPC disassembly during mitotic entry. Cell, 144(4), 539-550. doi:10.1016/j.cell.2011.01.012.

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アイテムのパーマリンク: https://hdl.handle.net/21.11116/0000-000F-62A5-8 版のパーマリンク: https://hdl.handle.net/21.11116/0000-000F-62A6-7
資料種別: 学術論文

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 作成者:
Laurell, E, 著者
Beck, K, 著者
Krupina, K, 著者
Theerthagiri, G1, 著者           
Bodenmiller, B, 著者
Horvath, P, 著者
Aebersold, R, 著者
Antonin, W1, 著者                 
Kutay, U, 著者
所属:
1Antonin Group, Friedrich Miescher Laboratory, Max Planck Society, ou_3375712              

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 要旨: Disassembly of nuclear pore complexes (NPCs) is a decisive event during mitotic entry in cells undergoing open mitosis, yet the molecular mechanisms underlying NPC disassembly are unknown. Using chemical inhibition and depletion experiments we show that NPC disassembly is a phosphorylation-driven process, dependent on CDK1 activity and supported by members of the NIMA-related kinase (Nek) family. We identify phosphorylation of the GLFG-repeat nucleoporin Nup98 as an important step in mitotic NPC disassembly. Mitotic hyperphosphorylation of Nup98 is accomplished by multiple kinases, including CDK1 and Neks. Nuclei carrying a phosphodeficient mutant of Nup98 undergo nuclear envelope breakdown slowly, such that both the dissociation of Nup98 from NPCs and the permeabilization of the nuclear envelope are delayed. Together, our data provide evidence for a phosphorylation-dependent mechanism underlying disintegration of NPCs during prophase. Moreover, we identify mitotic phosphorylation of Nup98 as a rate-limiting step in mitotic NPC disassembly.

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 日付: 2011-02
 出版の状態: 出版
 ページ: -
 出版情報: -
 目次: -
 査読: -
 識別子(DOI, ISBNなど): DOI: 10.1016/j.cell.2011.01.012
PMID: 21335236
 学位: -

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出版物 1

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出版物名: Cell
種別: 学術雑誌
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出版社, 出版地: Cambridge, Mass. : Cell Press
ページ: - 巻号: 144 (4) 通巻号: - 開始・終了ページ: 539 - 550 識別子(ISBN, ISSN, DOIなど): ISSN: 0092-8674
CoNE: https://pure.mpg.de/cone/journals/resource/954925463183