Deutsch
 
Hilfe Datenschutzhinweis Impressum
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT
Zur Ablage hinzufügen
 Lokale TagsFreigabegeschichteDetailsÜbersicht
  The red alga Porphyridium as a host for molecular farming: Efficient production of immunologically active hepatitis C virus glycoprotein

Hammel, A., Cucos, L.-M., Caras, I., Ionescu, I., Tucureanu, C., Tofan, V., et al. (2024). The red alga Porphyridium as a host for molecular farming: Efficient production of immunologically active hepatitis C virus glycoprotein. PNAS, 121(24): e2400145121. doi:10.1073/pnas.2400145121.

Item is

 

einblenden: alle ausblenden: alle

Basisdaten

einblenden: ausblenden:
Datensatz-Permalink: https://hdl.handle.net/21.11116/0000-000F-666B-7 Versions-Permalink: https://hdl.handle.net/21.11116/0000-000F-666D-5
Genre: Zeitschriftenartikel
Alternativer Titel : Proceedings of the National Academy of Sciences

Externe Referenzen

einblenden:

Urheber

einblenden:
ausblenden:
 Urheber:
Hammel, A.1, Autor                 
Cucos, Lia-Maria2, Autor
Caras, Iuliana2, Autor
Ionescu, Irina2, Autor
Tucureanu, Catalin2, Autor
Tofan, Vlad2, Autor
Costache, Adriana2, Autor
Onu, Adrian2, Autor
Hoepfner, Lara2, Autor
Hippler, Michael2, Autor
Neupert, J.1, Autor                 
Popescu, Costin-Ioan2, Autor
Stavaru, Crina2, Autor
Branza-Nichita, Norica2, Autor
Bock, R.1, Autor                 
Affiliations:
1Organelle Biology and Biotechnology, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753326              
2external, ou_persistent22              

Inhalt

einblenden:
ausblenden:
Schlagwörter: -
 Zusammenfassung: Microalgae are promising production platforms for the cost-effective production of recombinant proteins. We have recently established that the red alga Porphyridium purpureum provides superior transgene expression properties, due to the episomal maintenance of transformation vectors as multicopy plasmids in the nucleus. Here, we have explored the potential of Porphyridium to synthesize complex pharmaceutical proteins to high levels. Testing expression constructs for a candidate subunit vaccine against the hepatitis C virus (HCV), we show that the soluble HCV E2 glycoprotein can be produced in transgenic algal cultures to high levels. The antigen undergoes faithful posttranslational modification by N-glycosylation and is recognized by conformationally selective antibodies, suggesting that it adopts a proper antigenic conformation in the endoplasmic reticulum of red algal cells. We also report the experimental determination of the structure of the N-glycan moiety that is attached to glycosylated proteins in Porphyridium. Finally, we demonstrate the immunogenicity of the HCV antigen produced in red algae when administered by injection as pure protein or by feeding of algal biomass.

Details

einblenden:
ausblenden:
Sprache(n): eng - English
 Datum: 2024-06-042024-06
 Publikationsstatus: Erschienen
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: -
 Identifikatoren: DOI: 10.1073/pnas.2400145121
 Art des Abschluß: -

Veranstaltung

einblenden:

Entscheidung

einblenden:

Projektinformation

einblenden:

Quelle 1

einblenden:
ausblenden:
Titel: PNAS
  Andere : Proceedings of the National Academy of Sciences of the United States of America
  Andere : Proceedings of the National Academy of Sciences of the USA
  Kurztitel : Proc. Natl. Acad. Sci. U. S. A.
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: Washington, D.C. : National Academy of Sciences
Seiten: - Band / Heft: 121 (24) Artikelnummer: e2400145121 Start- / Endseite: - Identifikator: ISSN: 0027-8424
CoNE: https://pure.mpg.de/cone/journals/resource/954925427230