Deutsch
 
Hilfe Datenschutzhinweis Impressum
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT
Zur Ablage hinzufügen
 Lokale TagsFreigabegeschichteDetailsÜbersicht
  Power Density Titration of Reversible Photoisomerization of a Fluorescent Protein Chromophore in the Presence of Thermally Driven Barrier Crossing Shown by Quantitative Millisecond Serial Synchrotron X-ray Crystallography

Baxter, J. M., Hutchison, C. D. M., Fadini, A., Maghlaoui, K., Cordon-Preciado, V., Morgan, R. M. L., et al. (2024). Power Density Titration of Reversible Photoisomerization of a Fluorescent Protein Chromophore in the Presence of Thermally Driven Barrier Crossing Shown by Quantitative Millisecond Serial Synchrotron X-ray Crystallography. Journal of the American Chemical Society, 146(24), 16394-16403. doi:10.1021/jacs.3c12883.

Item is

 

einblenden: alle ausblenden: alle

Basisdaten

einblenden: ausblenden:
Datensatz-Permalink: https://hdl.handle.net/21.11116/0000-000F-6DFB-D Versions-Permalink: https://hdl.handle.net/21.11116/0000-000F-7262-2
Genre: Zeitschriftenartikel

Dateien

einblenden: Dateien
ausblenden: Dateien
:
ja3c12883_si_001.pdf (Ergänzendes Material), 793KB
Öffnen   Speichern
Datei-Permalink:
https://hdl.handle.net/21.11116/0000-000F-6DFE-A
Name:
ja3c12883_si_001.pdf
Beschreibung:
Supporting Information:
OA-Status:
Keine Angabe
Sichtbarkeit:
Öffentlich
MIME-Typ / Prüfsumme:
application/pdf / [MD5]
Technische Metadaten:
Öffnen
Copyright Datum:
-
Copyright Info:
High-resolution SSX power titration chromophore-omit maps, occupancy fitting, crystallographic data tables, modeling of laser and X-ray heating, absorption spectra, flash photolysis error propagation and modeling, knife-edge fitting. Coordinates and structure factor amplitudes have been deposited in the PDB database. For preconverted (Trans) rsKiiro under different 400 nm illumination conditions: 0 mJ/mm2 (PDB 8UL0), 0.1 mJ/mm2 (PDB 8UL1), 0.53 mJ/mm2 (PDB 8UL2), 1.78 mJ/mm2 (PDB 8UL3), 6.74 mJ/mm2(PDB 8UL4), and 14.43 mJ/mm2 (PDB 8UL5)
Lizenz:
-
:
baxter-et-al-2024-power-density-titration-of-reversible-photoisomerization-of-a-fluorescent-protein-chromophore-in-the.pdf (Verlagsversion), 6MB
Öffnen   Speichern
Datei-Permalink:
https://hdl.handle.net/21.11116/0000-000F-7263-1
Name:
baxter-et-al-2024-power-density-titration-of-reversible-photoisomerization-of-a-fluorescent-protein-chromophore-in-the.pdf
Beschreibung:
-
OA-Status:
Hybrid
Sichtbarkeit:
Öffentlich
MIME-Typ / Prüfsumme:
application/pdf / [MD5]
Technische Metadaten:
Öffnen
Copyright Datum:
2024
Copyright Info:
© The Authors. Published by American Chemical Society
Lizenz:
https://creativecommons.org/licenses/by/4.0/

Externe Referenzen

einblenden:
ausblenden:
externe Referenz:
https://doi.org/10.1021/jacs.3c12883 (Verlagsversion)
Beschreibung:
-
OA-Status:
Hybrid

Urheber

einblenden:
ausblenden:
 Urheber:
Baxter, J. M.1, Autor
Hutchison, C. D. M.1, Autor
Fadini, A.1, Autor
Maghlaoui, K.1, Autor
Cordon-Preciado, V.1, Autor
Morgan, R. M. L.2, Autor
Agthe, M.3, Autor
Horrell, S.4, Autor
Tellkamp, F.5, Autor           
Mehrabi, P.6, Autor           
Pfeifer, Y.7, Autor
Müller-Werkmeister, H. M.7, Autor
von Stetten, D.3, Autor
Pearson, A. R.8, Autor
van Thor, J. J.1, Autor
Affiliations:
1Department of Life Sciences, Imperial College London, ou_persistent22              
2Center for Structural Biology, Imperial College London, ou_persistent22              
3European Molecular Biology Laboratory (EMBL), ou_persistent22              
4Department of Physics, Center for Free-Electron Laser Science, Institute for Nanostructure and Solid State Physics, University of Hamburg, ou_persistent22              
5Machine Physics, Scientific Service Units, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society, ou_2074322              
6Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society, ou_1938288              
7Institute of Chemistry-Physical Chemistry, University of Potsdam,, ou_persistent22              
8Institute for Nanostructure and Solid State Physics & The Hamburg Centre for Ultrafast Imaging, HARBOR, Universität Hamburg, ou_persistent22              

Inhalt

einblenden:
ausblenden:
Schlagwörter: -
 Zusammenfassung: We present millisecond quantitative serial X-ray crystallography at 1.7 Å resolution demonstrating precise optical control of reversible population transfer from Trans–Cis and Cis–Trans photoisomerization of a reversibly switchable fluorescent protein, rsKiiro. Quantitative results from the analysis of electron density differences, extrapolated structure factors, and occupancy refinements are shown to correspond to optical measurements of photoinduced population transfer and have sensitivity to a few percent in concentration differences. Millisecond time-resolved concentration differences are precisely and reversibly controlled through intense continuous wave laser illuminations at 405 and 473 nm for the Trans-to-Cis and Cis-to-Trans reactions, respectively, while the X-ray crystallographic measurement and laser illumination of the metastable Trans chromophore conformation causes partial thermally driven reconversion across a 91.5 kJ/mol thermal barrier from which a temperature jump between 112 and 128 K is extracted.

Details

einblenden:
ausblenden:
Sprache(n): eng - English
 Datum: 2024-05-282023-11-162024-05-292024-06-072024-06-19
 Publikationsstatus: Erschienen
 Seiten: 10
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: DOI: 10.1021/jacs.3c12883
 Art des Abschluß: -

Veranstaltung

einblenden:

Entscheidung

einblenden:

Projektinformation

einblenden: ausblenden:
Projektname : J.J.v.T. acknowledges support by the Biotechnology and Biological Sciences Research Council (BBSRC) [BB/P00752X/1]. A.R.P. is supported by the Clusters of Excellence “CUI: Advanced Imaging of Matter” of the Deutsche Forschungsgemeinschaft (DFG)─EXC 2056─project ID 390715994 and “The Hamburg Centre for Ultrafast Imaging”─EXC 1074─project ID 194651731. The authors acknowledge support from Imperial College London Centre for Structural Biology, the CCP4/DLS 2018 workshop, the Dynamic Structural Biology BAG as part of the UK XFEL hub, the DESY Beamline P11, BAG-20170381, and EMBL P14.2 BAG MX-660. T-REXX is supported by the Bundesministerium für Bildung und Forschung (Verbundforschungsprojekte 05K16GU1, 05K19GU1 & 05K22GU6). H.M.W. is supported by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation under Germany’s Excellence Strategy (EXC 2008/1-390540038, UniSysCat). The authors would like to acknowledge Prof. Nils Huse, Universität Hamburg, for the loan of equipment used in the SSX experiments.
Grant ID : -
Förderprogramm : -
Förderorganisation : -

Quelle 1

einblenden:
ausblenden:
Titel: Journal of the American Chemical Society
  Andere : JACS
  Kurztitel : J. Am. Chem. Soc.
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: Washington, DC : American Chemical Society
Seiten: - Band / Heft: 146 (24) Artikelnummer: - Start- / Endseite: 16394 - 16403 Identifikator: ISSN: 0002-7863
CoNE: https://pure.mpg.de/cone/journals/resource/954925376870