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  Thermodynamics of the formylmethanofuran dehydrogenase reaction in Methanobacterium thermoautotrophicum

Bertram, P. A., & Thauer, R. K. (1994). Thermodynamics of the formylmethanofuran dehydrogenase reaction in Methanobacterium thermoautotrophicum. European Journal of Biochemistry, 226(3), 811-818. doi:10.1111/j.1432-1033.1994.t01-1-00811.x.

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Genre: Journal Article
Alternative Title : European Journal of Biochemistry

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Bertram, Peter A.1, 2, Author
Thauer, Rudolf K.1, 2, Author                 
Affiliations:
1Department of Biochemistry, Alumni, Max Planck Institute for Terrestrial Microbiology, Max Planck Society, ou_3266311              
2Laboratorium für Mikrobiologie, Fachbereich Biologie, Philipps- Universität, Marburg, ou_persistent22              

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 Abstract: Purified formylmethanofuran dehydrogenase from Methanobacterium thermoautotrophicum, which is a thermophilic methanogenic Archaeon growing on H2 and CO2, was shown to catalyze the reversible reduction of CO2 to N-formylmethanofuran with 1,1?,2,2?-tetramethylviologen (E?o=? 550 mV) as electron donor. The rate of CO2 reduction was approximately 25 times higher than the rate of N-formylmethanofuran dehydrogenation. From determinations of equilibrium concentrations at 60°C and pH 7.0 a midpoint potential (E?o) for the CO2+ methanofuran/formylmethanofuran couple of approximately ? 530 mV was estimated. The initial step of methanogenesis from CO2 thus has a midpoint potential considerably more negative than that of the H+/H2 couple (E?o=?460 mV at 60°C). Evidence is described indicating that the as-yet unidentified physiological electron donor of the formylmethanofuran dehydrogenase is present in the soluble cell fraction.

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Language(s): eng - English
 Dates: 1994-12
 Publication Status: Issued
 Pages: -
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 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: European Journal of Biochemistry
  Other : European Journal of Biochemistry and from 2005: The FEBS Journal
  Abbreviation : Eur. J. Biochem.
Source Genre: Journal
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Publ. Info: Wiley-Blackwell, England
Pages: - Volume / Issue: 226 (3) Sequence Number: - Start / End Page: 811 - 818 Identifier: ISSN: 1742-4658
CoNE: https://pure.mpg.de/cone/journals/resource/1742-4658