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  Studies on the catalytic mechanism of H2-forming methylenetetrahydromethanopterin dehydrogenase: para-ortho H2 conversion rates in H2O and D2O

Hartmann, G. C., Santamaria, E., Fernández, V. M., & Thauer, R. K. (1996). Studies on the catalytic mechanism of H2-forming methylenetetrahydromethanopterin dehydrogenase: para-ortho H2 conversion rates in H2O and D2O. Journal of Biological Inorganic Chemistry, 1(5), 446-450. doi:10.1007/s007750050077.

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https://doi.org/10.1007/s007750050077 (Publisher version)
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 Creators:
Hartmann, Gudrun C.1, 2, Author
Santamaria, Elena3, Author
Fernández, Victor M.3, Author
Thauer, Rudolf K.1, 2, Author                 
Affiliations:
1Department of Biochemistry, Alumni, Max Planck Institute for Terrestrial Microbiology, Max Planck Society, ou_3266311              
2Laboratorium für Mikrobiologie, Fachbereich Biologie, Philipps- Universität, Marburg, ou_persistent22              
3external, ou_persistent22              

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 Abstract:  H2–forming N5,N10–methylenetetrahydromethanopterin dehydrogenase is a novel type of hydrogenase that contains neither nickel nor iron-sulfur clusters. Evidence has been presented that the reaction mechanism catalyzed by the enzyme is very similar to that of the formation of carbocations and H2 from alkanes under superacidic conditions. We present here further results in support of this mechanism. It was found that the purified enzyme per se did not catalyze the conversion of para H2 to ortho H2, a reaction catalyzed by all other hydrogenases known to date. However, it catalyzed the conversion in the presence of the substrate N5,N10–methenyltetrahydromethanopterin (CH≡H4MPT+), indicating that for heterolytic cleavage of H2 the enzyme-CH≡H4MPT+ complex is required. In D2O, the formation of HD and D2 from H2 rather than a para–ortho H2 conversion was observed, indicating that after heterolytic cleavage of H2 the dissociation of the proton from the enzyme-substrate complex is fast relative to the re-formation of free H2.

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Language(s): eng - English
 Dates: 1996-10
 Publication Status: Issued
 Pages: -
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: URI: https://doi.org/10.1007/s007750050077
Other: Hartmann1996
DOI: 10.1007/s007750050077
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Title: Journal of Biological Inorganic Chemistry
  Abbreviation : J. Biol. Inorg. Chem.
Source Genre: Journal
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Publ. Info: Berlin : Springer
Pages: - Volume / Issue: 1 (5) Sequence Number: - Start / End Page: 446 - 450 Identifier: ISSN: 0949-8257
CoNE: https://pure.mpg.de/cone/journals/resource/954925573943