English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
 
 
DownloadE-Mail
 DetailsSummary
  Chloroplast Cell-Free Systems from Different Plant Species as a Rapid Prototyping Platform

Böhm, C. V., Inckemann, R., Burgis, M., Baumann, J., Brinkmann, C. K., Lipinska, K. E., et al. (2024). Chloroplast Cell-Free Systems from Different Plant Species as a Rapid Prototyping Platform. ACS Synthetic Biology. doi:10.1021/acssynbio.4c00117.

Item is

 

show all hide all

Basic

show hide
Item Permalink: https://hdl.handle.net/21.11116/0000-000F-976F-B Version Permalink: https://hdl.handle.net/21.11116/0000-000F-9770-8
Genre: Journal Article
Alternative Title : ACS Synthetic Biology

Files

show Files

Locators

show
hide
Locator:
https://doi.org/10.1021/acssynbio.4c00117 (Publisher version)
Description:
-
OA-Status:
Hybrid

Creators

show
hide
 Creators:
Böhm, Clemens V.1, Author
Inckemann, René1, Author
Burgis, Michael2, Author
Baumann, Jessica2, Author
Brinkmann, Cedric K.1, Author
Lipinska, Katarzyna E.1, Author
Gilles, Sara1, Author
Freudigmann, Jonas2, Author
Seiler, Vinca N.2, Author
Clark, Lauren G.2, Author
Jewett, Michael C.2, Author
Voll, Lars M.2, Author
Niederholtmeyer, Henrike1, Author
Affiliations:
1Max Planck Institute for Terrestrial Microbiology_others, Max Planck Institute for Terrestrial Microbiology, Max Planck Society, Karl-von Frisch Str. 10, 35043 Marburg, Germany, ou_3556424              
2external, ou_persistent22              

Content

show
hide
Free keywords: -
 Abstract: Climate change poses a significant threat to global agriculture, necessitating innovative solutions. Plant synthetic biology, particularly chloroplast engineering, holds promise as a viable approach to this challenge. Chloroplasts present a variety of advantageous traits for genetic engineering, but the development of genetic tools and genetic part characterization in these organelles is hindered by the lengthy time scales required to generate transplastomic organisms. To address these challenges, we have established a versatile protocol for generating highly active chloroplast-based cell-free gene expression (CFE) systems derived from a diverse range of plant species, including wheat (monocot), spinach, and poplar trees (dicots). We show that these systems work with conventionally used T7 RNA polymerase as well as the endogenous chloroplast polymerases, allowing for detailed characterization and prototyping of regulatory sequences at both transcription and translation levels. To demonstrate the platform for characterization of promoters and 5′ and 3′ untranslated regions (UTRs) in higher plant chloroplast gene expression, we analyze a collection of 23 5′UTRs, 10 3′UTRs, and 6 chloroplast promoters, assessed their expression in spinach and wheat extracts, and found consistency in expression patterns, suggesting cross-species compatibility. Looking forward, our chloroplast CFE systems open new avenues for plant synthetic biology, offering prototyping tools for both understanding gene expression and developing engineered plants, which could help meet the demands of a changing global climate.

Details

show
hide
Language(s): eng - English
 Dates: 2024-07-19
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: URI: https://doi.org/10.1021/acssynbio.4c00117
DOI: 10.1021/acssynbio.4c00117
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: ACS Synthetic Biology
  Abbreviation : ACS Synth. Biol.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Washington, D.C. : American Chemical Society
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: - Identifier: ISSN: 2161-5063
CoNE: https://pure.mpg.de/cone/journals/resource/2161-5063