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  Simultaneous multicolor fluorescence imaging using PSF splitting

Van den Eynde, R., Hertel, F., Abakumov, S., Krajnik, B., Hugelier, S., Auer, A., et al. (2024). Simultaneous multicolor fluorescence imaging using PSF splitting. Nature Methods. doi:10.1038/s41592-024-02383-7.

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Item Permalink: https://hdl.handle.net/21.11116/0000-000F-DDBA-7 Version Permalink: https://hdl.handle.net/21.11116/0000-000F-DDBB-6
Genre: Journal Article

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s41592-024-02383-7.pdf (Publisher version), 3MB
 
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 Creators:
Van den Eynde, Robin, Author
Hertel, Fabian, Author
Abakumov, Sergey, Author
Krajnik, Bartosz, Author
Hugelier, Siewert, Author
Auer, Alexander, Author
Hellmeier, Joschka, Author
Schlichthaerle, Thomas, Author
Grattan, Rachel M., Author
Lidke, Diane S., Author
Jungmann, Ralf, Author
Leutenegger, Marcel1, Author           
Vandenberg, Wim, Author
Dedecker, Peter, Author
Affiliations:
1Department of NanoBiophotonics, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society, ou_3350048              

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 Abstract: We present a way to encode more information in fluorescence imaging by splitting the original point spread function (PSF), which offers broadband operation and compatibility with other PSF engineering modalities and existing analysis tools. We demonstrate the approach using the ‘Circulator’, an add-on that encodes the fluorophore emission band into the PSF, enabling simultaneous multicolor super-resolution and single-molecule microscopy using essentially the full field of view.

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Language(s): eng - English
 Dates: 2024-09-06
 Publication Status: Published online
 Pages: -
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1038/s41592-024-02383-7
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Project name : NanoCellActivity
Grant ID : 714688
Funding program : Horizon 2020 (H2020)
Funding organization : European Commission (EC)

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Title: Nature Methods
  Abbreviation : Nat Methods
Source Genre: Journal
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Publ. Info: New York, NY : Nature Publishing Group
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: - Identifier: ISSN: 1548-7091
CoNE: https://pure.mpg.de/cone/journals/resource/111088195279556