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  The mitochondrial citrate synthase from Tetrahymena thermophila does not form an intermediate filament

Lometto, S., Sparvoli, D., Malengo, G., Heimerl, T., & Hochberg, G. K. A. (2024). The mitochondrial citrate synthase from Tetrahymena thermophila does not form an intermediate filament. European Journal of Protistology, 96: 126121. doi:10.1016/j.ejop.2024.126121.

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https://doi.org/10.1016/j.ejop.2024.126121 (Publisher version)
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 Creators:
Lometto, Stefano1, Author           
Sparvoli, Daniela2, Author
Malengo, Gabriele3, Author           
Heimerl, Thomas2, Author
Hochberg, Georg K. A.1, Author                 
Affiliations:
1Max Planck Research Group Evolutionary Biochemistry, Max Planck Institute for Terrestrial Microbiology, Max Planck Society, ou_3266300              
2external, ou_persistent22              
3Core Facility Flow Cytometry and Imaging, Max Planck Institute for Terrestrial Microbiology, Max Planck Society, ou_3265893              

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Free keywords: Citrate synthase, , Intermediate filament, Conjugation, MES buffer
 Abstract: The mitochondrial citrate synthase (mCS) purified from the ciliate Tetrahymena thermophila has been reported to form intermediate-filament-like structures during conjugation and to self-assemble into fibers when recombinantly expressed. This would represent a rare example of a tractable and recent origin of a novel cytoskeletal element. In an attempt to investigate the evolutionary emergence of this behavior, we re-investigated the ability of Tetrahymena’s mCS to form filaments in vivo. Using strep-tagged mCS in Tetrahymena and monoclonal antibodies, we found no evidence of filamentous structures during conjugation or starvation. Extensive biochemical characterization of mCS revealed that the self-assembly of recombinant protein is triggered by a specific chemical moiety shared by MES and HEPES buffers used in previous studies. The absence of indicative phenotypes in fiber-deficient GFP-tagged mutants indicates that Tetrahymena mCS did not evolve a structural role in sexual reproduction or metabolic regulation.

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Language(s): eng - English
 Dates: 2024-10-11
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: European Journal of Protistology
Source Genre: Journal
 Creator(s):
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Publ. Info: Elsevier
Pages: - Volume / Issue: 96 Sequence Number: 126121 Start / End Page: - Identifier: ISSN: 0932-4739
CoNE: https://pure.mpg.de/cone/journals/resource/954928506596