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  Light-mediated discovery of surfaceome nanoscale organization and intercellular receptor interaction networks

Müller, M., Gräbnitz, F., Barandun, N., Shen, Y., Wendt, F., Steiner, S. N., et al. (2021). Light-mediated discovery of surfaceome nanoscale organization and intercellular receptor interaction networks. Nature Communications, 12: 7036, pp. 1-17. doi:10.1038/s41467-021-27280-x.

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Item Permalink: https://hdl.handle.net/21.11116/0000-000F-F41A-1 Version Permalink: https://hdl.handle.net/21.11116/0000-000F-F41B-0
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Creators

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 Creators:
Müller, Maik, Author
Gräbnitz, Fabienne, Author
Barandun, Niculò, Author
Shen, Yang, Author
Wendt, Fabian, Author
Steiner, Sebastian N., Author
Severin, Yannik, Author
Vetterli, Stefan U., Author
Mondal, Milon, Author
Prudent, James R., Author
Hofmann, Raphael, Author
van Oostrum, Marc, Author
Sarott, Roman C.1, Author           
Nesvizhskii, Alexey I., Author
Carreira, Erick M., Author
Bode, Jeffrey W., Author
Snijder, Berend, Author
Robinson, John A., Author
Loessner, Martin J., Author
Oxenius, Annette, Author
Wollscheid, Bernd, Author more..
Affiliations:
1Chemical Biology, Max Planck Institute for Medical Research, Max Planck Society, ou_2364732              

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 Abstract: The molecular nanoscale organization of the surfaceome is a fundamental regulator of cellular signaling in health and disease. Technologies for mapping the spatial relationships of cell surface receptors and their extracellular signaling synapses would unlock theranostic opportunities to target protein communities and the possibility to engineer extracellular signaling. Here, we develop an optoproteomic technology termed LUX-MS that enables the targeted elucidation of acute protein interactions on and in between living cells using light-controlled singlet oxygen generators (SOG). By using SOG-coupled antibodies, small molecule drugs, biologics and intact viral particles, we demonstrate the ability of LUX-MS to decode ligand receptor interactions across organisms and to discover surfaceome receptor nanoscale organization with direct implications for drug action. Furthermore, by coupling SOG to antigens we achieved light-controlled molecular mapping of intercellular signaling within functional immune synapses between antigen-presenting cells and CD8+ T cells providing insights into T cell activation with spatiotemporal specificity. LUX-MS based decoding of surfaceome signaling architectures thereby provides a molecular framework for the rational development of theranostic strategies.

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Language(s): eng - English
 Dates: 2020-11-042021-11-092021-12-02
 Publication Status: Published online
 Pages: 17
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: URI: https://pubmed.ncbi.nlm.nih.gov/34857745/
URI: https://pmc.ncbi.nlm.nih.gov/articles/PMC8639842/
DOI: 10.1038/s41467-021-27280-x
 Degree: -

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Title: Nature Communications
  Abbreviation : Nat. Commun.
Source Genre: Journal
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Publ. Info: London : Nature Publishing Group
Pages: - Volume / Issue: 12 Sequence Number: 7036 Start / End Page: 1 - 17 Identifier: ISSN: 2041-1723
CoNE: https://pure.mpg.de/cone/journals/resource/2041-1723