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  Probing the modulation of enzyme kinetics by multi-temperature, time-resolved serial crystallography

Schulz, E.-C., Prester, A., von Stetten, D., Gore, G., Hatton, C. E., Bartels, K., et al. (2024). Probing the modulation of enzyme kinetics by multi-temperature, time-resolved serial crystallography. doi:10.1101/2021.11.07.467596.

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Item Permalink: https://hdl.handle.net/21.11116/0000-000F-FB2B-7 Version Permalink: https://hdl.handle.net/21.11116/0000-000F-FB2C-6
Genre: Preprint

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2021.11.07.467596v3.full.pdf (Preprint), 18MB
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2024
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https://doi.org/10.1101/2021.11.07.467596 (Preprint)
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 Creators:
Schulz, E.-C.1, 2, 3, Author           
Prester, A.1, Author
von Stetten, D.4, Author
Gore, G.1, Author
Hatton, C. E.3, Author
Bartels, K.1, Author
Leimkohl, J.-P.5, Author           
Schikora, H.5, Author           
Ginn, H. M.6, Author
Tellkamp, F.5, Author           
Mehrabi, P.2, 3, Author           
Affiliations:
1University Medical Center Hamburg-Eppendorf (UKE), ou_persistent22              
2Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society, ou_1938288              
3Institute for Nanostructure and Solid State Physics, University of Hamburg, ou_persistent22              
4European Molecular Biology Laboratory (EMBL), ou_persistent22              
5Machine Physics, Scientific Service Units, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society, ou_2074322              
6Deutsches Elektronen Synchrotron (DESY), ou_persistent22              

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 Abstract: We present an environmental enclosure for fixed-target serial crystallography, enabling X-ray diffraction experiments in a temperature window from below 10 °C to above 70 °C - a universal parameter of protein function. Via 5D-SSX time-resolved experiments can now be carried out at physiological temperatures, providing fundamentally new insights into protein function. We show temperature-dependent modulation of turnover kinetics for the mesophilic β-lactamase CTX-M-14 and for the hyperthermophilic enzyme xylose isomerase.

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Language(s): eng - English
 Dates: 2024-07-24
 Publication Status: Published online
 Pages: 48
 Publishing info: -
 Table of Contents: -
 Rev. Type: No review
 Identifiers: DOI: 10.1101/2021.11.07.467596
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