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  Engineering modular and tunable single-molecule sensors by decoupling sensing from signal output

Grabenhorst, L., Pfeiffer, M., Schinkel, T., Kümmerlin, M., Brüggenthies, G. A., Maglic, J. B., et al. (2025). Engineering modular and tunable single-molecule sensors by decoupling sensing from signal output. Nature Nanotechnology, 20(2), 303-310. doi:10.1038/s41565-024-01804-0.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0010-D3DC-8 Version Permalink: https://hdl.handle.net/21.11116/0000-0010-D3DD-7
Genre: Journal Article

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 Creators:
Grabenhorst, Lennart, Author
Pfeiffer, Martina, Author
Schinkel, Thea, Author
Kümmerlin, Mirjam, Author
Brüggenthies, Gereon A., Author
Maglic, Jasmin B., Author
Selbach, Florian, Author
Murr, Alexander T., Author
Tinnefeld, Philip, Author
Glembockyte, Viktorija1, Author           
Affiliations:
1Max Planck Institute for Medical Research, Max Planck Society, Jahnstrasse 29, 69120 Heidelberg, DE, ou_1125545              

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 Abstract: Biosensors play key roles in medical research and diagnostics. However, the development of biosensors for new biomolecular targets of interest often involves tedious optimization steps to ensure a high signal response at the analyte concentration of interest. Here we show a modular nanosensor platform that facilitates these steps by offering ways to decouple and independently tune the signal output as well as the response window. Our approach utilizes a dynamic DNA origami nanostructure to engineer a high optical signal response based on fluorescence resonance energy transfer. We demonstrate mechanisms to tune the sensor's response window, specificity and cooperativity as well as highlight the modularity of the proposed platform by extending it to different biomolecular targets including more complex sensing schemes. This versatile nanosensor platform offers a promising starting point for the rapid development of biosensors with tailored properties.

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Language(s): eng - English
 Dates: 2023-11-232024-09-122024-11-072025-02
 Publication Status: Issued
 Pages: 11
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: URI: https://pubmed.ncbi.nlm.nih.gov/39511326/
DOI: 10.1038/s41565-024-01804-0
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Title: Nature Nanotechnology
  Other : Nat. Nanotechnol.
Source Genre: Journal
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Publ. Info: London : Nature Publishing Group
Pages: - Volume / Issue: 20 (2) Sequence Number: - Start / End Page: 303 - 310 Identifier: ISSN: 1748-3387
CoNE: https://pure.mpg.de/cone/journals/resource/1000000000239770