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Free keywords:
Saccharomyces-cerevisiae; sphingosine bases; higher-plants; fatty-acids; biosynthesis; desaturase; hydroxylation; identification; oxidation; probes
Abstract:
The stereochemical course of the dihydroceramide Delta(4)-(E)-desaturase from Candida albicans, cloned and expressed in the yeast Saccharomyces cerevisiae strain sur2Delta, was determined using stereospecifically labelled (2R,3S)-[2,3,4,4-H-2(4)]-palmit