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Schlagwörter:
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Zusammenfassung:
As the first identified scorpion toxin active on both big conductance Ca2+-activated K+ channels (BK) and small
conductance Ca2+-activated K+ channels (SK), BmBKTx1 has
been proposed to have two separate functional faces for two
targets. To investigate this hypothesis, two double mutants,
K21A-Y30A and R9A-K11A, together with wild-type toxin
were expressed in Escherichia coli. The recombinant toxins
were tested on cockroach BK and rat SK2 channel for
functional assay. Mutant K21A-Y30A had a dramatic loss of
function on BK but retained its function on SK. Mutant
R9A-K11A did not lose function on BK or SK. These data
support the two functional-face hypothesis and indicate that
the BK face is on the C-terminal β-sheet.
