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  Use of real-time PCR for determining copy number and zygosity in transgenic plants

Bubner, B., & Baldwin, I. T. (2004). Use of real-time PCR for determining copy number and zygosity in transgenic plants. Plant Cell Reports, 23(5), 263-271. doi:10.1007/s00299-004-0859-y.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-0012-AC82-1 Version Permalink: https://hdl.handle.net/21.11116/0000-0009-3730-3
Genre: Journal Article

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ITB148.pdf (Publisher version), 0B
 
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 Creators:
Bubner, B.1, Author           
Baldwin, I. T.1, Author           
Affiliations:
1Department of Molecular Ecology, MPI for Chemical Ecology, Max Planck Society, ou_24029              

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Free keywords: Transgenic plants Real-time pcr Copy number Zygosity. Polymerase-chain-reaction Agrobacterium-mediated transformation Event-specific detection Quantitative pcr Detection system Competitive pcr Taqman assay Rapid method Green-i Expression. Plant Sciences in Current Contents(R)/Agricultural, Biology & Environmental Sciences. Animal & Plant Sciences in Current Contents(R)/Life Sciences.
 Abstract: This review examines how real-time PCR can be used to determine copy number and zygosity in transgenic plants. Distinguishing between plants that harbor one and two copies of a transgene or are hemizygous and homozygous requires the ability to routinely

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 Dates: 2004
 Publication Status: Issued
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 Identifiers: Other: ITB148
DOI: 10.1007/s00299-004-0859-y
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Title: Plant Cell Reports
Source Genre: Journal
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Publ. Info: Berlin : Springer
Pages: - Volume / Issue: 23 (5) Sequence Number: - Start / End Page: 263 - 271 Identifier: ISSN: 0721-7714
CoNE: https://pure.mpg.de/cone/journals/resource/954925532004