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  Detection and removal of an artefact fatty acid from the binding site of recombinant Bombyx mori pheromone-binding protein

Oldham, N. J., Krieger, J., Breer, H., & Svatos, A. (2001). Detection and removal of an artefact fatty acid from the binding site of recombinant Bombyx mori pheromone-binding protein. Chemical Senses, 26(5), 529-531. doi:10.1093/chemse/26.5.529.

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MS015.pdf (Publisher version), 0B
 
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http://dx.doi.org/10.1093/chemse/26.5.529 (Publisher version)
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 Creators:
Oldham, N. J.1, Author           
Krieger, J., Author
Breer, H., Author
Svatos, A.1, Author           
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1Research Group Mass Spectrometry, MPI for Chemical Ecology, Max Planck Society, ou_421899              

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Free keywords: Mass-spectrometry; purification; expression
 Abstract: Recombinant Bombyx mori pheromone-binding protein (PBP), purified from an Escherichia coil expression system, has been found to contain (11Z)-octadecenoic acid (cis-vaccenic acid) as an artefact ligand. An efficient delipidation procedure is described t

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 Dates: 2001
 Publication Status: Issued
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 Identifiers: Other: MS015
DOI: 10.1093/chemse/26.5.529
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Title: Chemical Senses
Source Genre: Journal
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Publ. Info: Oxford : Oxford University Press [etc.]
Pages: - Volume / Issue: 26 (5) Sequence Number: - Start / End Page: 529 - 531 Identifier: ISSN: 0379-864X
CoNE: https://pure.mpg.de/cone/journals/resource/954928560444