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Schlagwörter:
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Zusammenfassung:
Microstructured silicon wafers were employed as miniaturized solid-phase reaction vessels as well as miniaturized
micro titer plates. Employing piezoelectric drop-on-demand liquid jets, a combinatorial library of 256 Peptides
was synthesized on single beads. The synthesis protocol was associated to the location in the silicon nano-well
arrangement. Products were photolytically cleaved in the same well that was used for synthesis and subsequently
interrogated for thrombin inhibition in a homogeneous competition assay. The assay procedure was based on
drop-on-demand liquid delivery and laser induced fluorescence imaging. The novel format proved useful for the
integration of both synthesis and screening into one platform, a prerequisite for an iterative, evolutionary approach
towards drug discovery.
