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  Acid-base-driven matrix-assisted mass spectrometry for targeted metabolomics

Shroff, R., Rulíšek, L., Doubský, J., & Svatoš, A. (2009). Acid-base-driven matrix-assisted mass spectrometry for targeted metabolomics. Proceedings of the National Academy of Sciences of the United States of America, 106(25), 10092-10096. doi:10.1073/pnas.0900914106.

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Shroff, Rohit1, 2, Author           
Rulíšek, Lubomír, Author
Doubský, Jan1, Author           
Svatoš, Aleš1, Author           
Affiliations:
1Research Group Mass Spectrometry, MPI for Chemical Ecology, Max Planck Society, ou_421899              
2IMPRS on Ecological Interactions, MPI for Chemical Ecology, Max Planck Society, Jena, DE, ou_421900              

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 Abstract: The ability to charge huge biomolecules without breaking them apart has made matrix-assisted laser desorption/ionization (MALDI) mass spectrometry an indispensable tool for biomolecular analysis. Conventional, empirically selected matrices produce abundant matrix ion clusters in the low-mass region (<500 Da), hampering the application of MALDI-MS to metabolomics. An ionization mode of MAILD, a rational protocol for matrix selection based on Brønsted–Lowry acid–base theory and its application to metabolomics, biological screening/profiling/imaging, and clinical diagnostics is illustrated. Numerous metabolites, covering important metabolic pathways (Krebs' cycle, fatty acid and glucosinolate biosynthesis), were detected in extracts, biofluids, and/or in biological tissues (Arabidopsis thaliana, Drosophila melanogaster, Acyrthosiphon pisum, and human blood). This approach moves matrix selection from “black art” to rational design and sets a paradigm for small-molecule analysis via MALDI-MS.

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 Dates: 2009
 Publication Status: Published in print
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 Identifiers: DOI: 10.1073/pnas.0900914106
Other: MS109
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Title: Proceedings of the National Academy of Sciences of the United States of America
  Other : Proceedings of the National Academy of Sciences of the USA
  Other : Proc. Acad. Sci. U.S.A.
  Abbreviation : PNAS
Source Genre: Journal
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Publ. Info: Washington, D.C. : National Academy of Sciences
Pages: - Volume / Issue: 106 (25) Sequence Number: - Start / End Page: 10092 - 10096 Identifier: ISSN: 0027-8424
CoNE: https://pure.mpg.de/cone/journals/resource/954925427230