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  Nonuniform distribution of glucosinolates in Arabidopsis thaliana leaves has important consequences for plant defense

Shroff, R., Vergara, F., Muck, A., Svatoš, A., & Gershenzon, J. (2008). Nonuniform distribution of glucosinolates in Arabidopsis thaliana leaves has important consequences for plant defense. Proceedings of the National Academy of Sciences of the United States of America, 105, 6196-6201. doi:10.1073/pnas.0711730105.

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Shroff, R.1, 2, Author           
Vergara, Fredd2, 3, Author           
Muck, A.1, Author           
Svatoš, Aleš1, Author           
Gershenzon, J.3, Author           
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1Research Group Mass Spectrometry, MPI for Chemical Ecology, Max Planck Society, ou_421899              
2IMPRS on Ecological Interactions, MPI for Chemical Ecology, Max Planck Society, Jena, DE, ou_421900              
3Department of Biochemistry, Prof. J. Gershenzon, MPI for Chemical Ecology, Max Planck Society, Jena, DE, ou_421893              

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 Abstract: The spatial distribution of plant defenses within a leaf may be critical in explaining patterns of herbivory. The generalist lepidopteran larvae, Helicoverpa armigera (the cotton bollworm), avoided the midvein and periphery of Arabidopsis thaliana rosette leaves and fed almost exclusively on the inner lamina. This feeding pattern was attributed to glucosinolates because it was not evident in a myrosinase mutant that lacks the ability to activate glucosinolate defenses by hydrolysis. To measure the spatial distribution of glucosinolates in A. thaliana leaves at a fine scale, we constructed ion intensity maps from MALDI-TOF (matrix assisted laser desorption/ionization-time of flight) mass spectra. The major glucosinolates were found to be more abundant in tissues of the midvein and the periphery of the leaf than the inner lamina, patterns that were validated by HPLC analyses of dissected leaves. In addition, there were differences in the proportions of the three major glucosinolates in different leaf regions. Hence, the distribution of glucosinolates within the leaf appears to control the feeding preference of H. armigera larvae. The preferential allocation of glucosinolates to the periphery may play a key role in the defense of leaves by creating a barrier to the feeding of chewing herbivores that frequently approach leaves from the edge.

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 Dates: 2008
 Publication Status: Published in print
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 Identifiers: Other: MS122
DOI: 10.1073/pnas.0711730105
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Title: Proceedings of the National Academy of Sciences of the United States of America
  Other : Proc. Acad. Sci. USA
  Abbreviation : PNAS
Source Genre: Journal
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Publ. Info: Washington, D.C. : National Academy of Sciences
Pages: - Volume / Issue: 105 Sequence Number: - Start / End Page: 6196 - 6201 Identifier: ISSN: 0027-8424
CoNE: https://pure.mpg.de/cone/journals/resource/954925427230