English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Oxylipin channelling in Nicotiana attenuata: Lipoxygenase 2 supplies substrates for green leaf volatile production

Allmann, S., Halitschke, R., Schuurink, R. C., & Baldwin, I. T. (2010). Oxylipin channelling in Nicotiana attenuata: Lipoxygenase 2 supplies substrates for green leaf volatile production. Plant, Cell and Environment, 33(12), 2028-2040. doi:10.1111/j.1365-3040.2010.02203.x.

Item is

Files

show Files
hide Files
:
ITB287.pdf (Publisher version), 370KB
 
File Permalink:
-
Name:
ITB287.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Chemical Ecology, MJCO; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
ITB287s1.zip (Supplementary material), 429KB
 
File Permalink:
-
Name:
ITB287s1.zip
Description:
-
Visibility:
Restricted (Max Planck Institute for Chemical Ecology, MJCO; )
MIME-Type / Checksum:
application/zip
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Description:
OA

Creators

show
hide
 Creators:
Allmann, Silke1, 2, Author              
Halitschke, Rayko3, Author              
Schuurink, R. C., Author
Baldwin, Ian Thomas1, Author              
Affiliations:
1Department of Molecular Ecology, Prof. I. T. Baldwin, MPI for Chemical Ecology, Max Planck Society, ou_24029              
2IMPRS on Ecological Interactions, MPI for Chemical Ecology, Max Planck Society, Jena, DE, ou_421900              
3External Organizations, ou_persistent22              

Content

show
hide
Free keywords: -
 Abstract: Lipoxygenases (LOXs) are key enzymes in the biosynthesis of oxylipins, and catalyse the formation of fatty acid hydroperoxides (HPs), which represent the first committed step in the synthesis of metabolites that function as signals and defences in plants. HPs are the initial substrates for different branches of the oxylipin pathway, and some plant species may express different LOX isoforms that supply specific branches. Here, we compare isogenic lines of the wild tobacco Nicotiana attenuata with reduced expression of NaLOX2 (irlox2) or NaLOX3 (irlox3) to determine the role of these different LOX isoforms in supplying substrates for two different pathways: green leaf volatiles (GLVs) and jasmonic acid (JA). Reduced NaLOX2 expression strongly decreased the production of GLVs without influencing the formation of JA and JA-related secondary metabolites. Conversely, reduced NaLOX3 expression strongly decreased JA biosynthesis, without influencing GLV production. The temporal expression of NaLOX2 and NaLOX3 also differed after elicitation; NaLOX3 was rapidly induced, attaining highest transcript levels within 1 h after elicitation, whereas NaLOX2 transcripts reached maximum levels after 14 h. These results demonstrate that N. attenuata channels the flux of HPs through the activities of different LOXs, leading to different direct and indirect defence responses mediating the plant's herbivore resistance.

Details

show
hide
Language(s):
 Dates: 20102010-122010
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: Other: ITB287
DOI: 10.1111/j.1365-3040.2010.02203.x
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Plant, Cell and Environment
  Other : Plant, Cell & Environment
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Oxford, England : Blackwell Science
Pages: - Volume / Issue: 33 (12) Sequence Number: - Start / End Page: 2028 - 2040 Identifier: ISSN: 0140-7791
CoNE: https://pure.mpg.de/cone/journals/resource/954925471334