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  Probing secondary structures of spin-labeled RNA by pulsed EPR spectroscopy.

Sicoli, G., Wachowius, F., Bennati, M., & Hoebartner, C. (2010). Probing secondary structures of spin-labeled RNA by pulsed EPR spectroscopy. Angewandte Chemie International Edition, 49(36), 6443-6447. doi:10.1002/anie.201000713.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-D545-6 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-F272-8
Genre: Journal Article

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 Creators:
Sicoli, G.1, Author              
Wachowius, F., Author
Bennati, M.1, Author              
Hoebartner, C.2, Author              
Affiliations:
1Research Group of Electron Paramagnetic Resonance, MPI for biophysical chemistry, Max Planck Society, ou_578606              
2Research Group of Nucleic Acid Chemistry, MPI for biophysical chemistry, Max Planck Society, ou_578605              

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Free keywords: EPR spectroscopy; PELDOR; RNA; secondary structures; spin labeling
 Abstract: Tempo, TEMPO! Spin-labeled RNAs are readily prepared by postsynthetic modification of RNA. This approach provides access to paramagnetic oligonucleotides with position-specific probes in cytidine, adenosine, and guanosine nucleobases (CT, AT, GT, respectively). In this way alternative RNA secondary structures can be investigated by distance measurements using pulsed EPR spectroscopy.

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Language(s): eng - English
 Dates: 2010-07-212010-08-23
 Publication Status: Published in print
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 Rev. Method: Peer
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Title: Angewandte Chemie International Edition
Source Genre: Journal
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Pages: - Volume / Issue: 49 (36) Sequence Number: - Start / End Page: 6443 - 6447 Identifier: -