English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Exon definition complexes contain the tri-snRNP and can be directly converted into B-like precatalytic splicing complexes.

Schneider, M., Will, C. L., Anokhina, M., Tazi, J., Urlaub, H., & Lührmann, R. (2010). Exon definition complexes contain the tri-snRNP and can be directly converted into B-like precatalytic splicing complexes. Molecular Cell, 38(2), 223-235. doi:10.1016/j.molcel.2010.02.027.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-D5C1-C Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-F271-A
Genre: Journal Article

Files

show Files
hide Files
:
587713.pdf (Publisher version), 2MB
Name:
587713.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
587713-Suppl.pdf (Supplementary material), 529KB
Name:
587713-Suppl.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Creators

show
hide
 Creators:
Schneider, M.1, Author              
Will, C. L.1, Author              
Anokhina, M.1, Author              
Tazi, J., Author
Urlaub, H.2, Author              
Lührmann, R.1, Author              
Affiliations:
1Department of Cellular Biochemistry, MPI for biophysical chemistry, Max Planck Society, ou_578576              
2Research Group of Bioanalytical Mass Spectrometry, MPI for biophysical chemistry, Max Planck Society, ou_578613              

Content

show
hide
Free keywords: RNA; Proteins
 Abstract: The first step in splicing of pre-mRNAs with long introns is exon definition, where U1 and U2 snRNPs bind at opposite ends of an exon. After exon definition, these snRNPs must form a complex across the upstream intron to allow splicing catalysis. Exon definition and conversion of cross-exon to cross-intron spliceosomal complexes are poorly understood. Here we demonstrate that, in addition to U1 and U2 snRNPs, cross-exon complexes contain U4, U5, and U6 (which form the tri-snRNP). Tri-snRNP docking involves the formation of U2/U6 helix II. This interaction is stabilized by a 5′ splice site (SS)-containing oligonucleotide, which can bind the tri-snRNP and convert the cross-exon complex into a cross-intron, B-like complex. Our data suggest that the switch from cross-exon to cross-intron complexes can occur directly when an exon-bound tri-snRNP interacts with an upstream 5′SS, without prior formation of a cross-intron A complex, revealing an alternative spliceosome assembly pathway.

Details

show
hide
Language(s): eng - English
 Dates: 2010-04-222010-04-23
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Molecular Cell
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 38 (2) Sequence Number: - Start / End Page: 223 - 235 Identifier: -