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  Effects in 94 GHz orientation-selected PELDOR on a rigid pair of radicals with non-collinear axes.

Sicoli, G., Argirevic, T., Stubbe, J., Tkach, I., & Bennati, M. (2010). Effects in 94 GHz orientation-selected PELDOR on a rigid pair of radicals with non-collinear axes. Applied Magnetic Resonance, 37(1-4), 539-548. doi:10.1007/s00723-009-0094-3.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-D6F4-3 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-F273-6
Genre: Journal Article

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Sicoli, G.1, Author              
Argirevic, T.1, Author              
Stubbe, J., Author
Tkach, I.1, Author              
Bennati, M.1, Author              
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1Research Group of Electron Paramagnetic Resonance, MPI for biophysical chemistry, Max Planck Society, ou_578606              

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 Abstract: For aromatic organic radicals, pulsed electron-electron double resonance (PELDOR) experiments at high magnetic fields provide information not only about the distance between the paramagnetic species but also about their relative orientation. However, the three-dimensional biradical structure is encoded in a complex pattern of orientation-selected PELDOR traces and the execution of the experiment is generally aggravated by constraints posed by the available hardware and the intrinsically low modulation depth observed. We present a 94 GHz PELDOR experiment performed with a commercial spectrometer and probe heads that permit separation of pump and detection frequencies up to 150 MHz. The setup is employed to examine the orientation selections on a general case of rigid biradicals with non-collinear g axes. The interacting radicals, a tyrosyl radical (Y122·) located in the β2 subunit and an 3-aminotyrosyl radical (NH2Y731·) located in the α2 subunit, are generated by Escherichia coli ribonucleotide reductase with a 3-aminotyrosine (NH2Y) site specifically incorporated into α2 in the presence of cytidine 5′-diphosphate and adenosine 5′-triphosphate. The experimental designs as well as some characteristic features of the observed modulation pattern are discussed.

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Language(s): eng - English
 Dates: 2009-11-132010-01
 Publication Status: Published in print
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 Rev. Method: Peer
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Title: Applied Magnetic Resonance
Source Genre: Journal
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Pages: - Volume / Issue: 37 (1-4) Sequence Number: - Start / End Page: 539 - 548 Identifier: -