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  Differentiation of embryonic stem cells.

Mansouri, A., Fukumitsu, H., Schindehuette, J., & Krieglstein, K. (2009). Differentiation of embryonic stem cells. Current Protocols in Neuroscience, 47(Unit 3.6), 3.6.1-3.6.21. doi:10.1002/0471142301.ns0306s47.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-D89A-E Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-F26A-B
Genre: Journal Article

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588140.pdf (Publisher version), 259KB
 
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 Creators:
Mansouri, A.1, Author              
Fukumitsu, H., Author
Schindehuette, J.1, Author              
Krieglstein, K., Author
Affiliations:
1Research Group of Molecular Cell Differentiation, MPI for biophysical chemistry, Max Planck Society, ou_578588              

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Free keywords: embryonic stem cells; dopaminergic neurons; SDIA; neural stem cells
 Abstract: Mouse embryonic stem (ES) cells are derived from mouse blastocyst and are able to generate all embryonic tissues in vitro. This propensity of ES cells has acquired considerable attention in recent years due to the promising potential for future cell replacement–based therapies. Therefore, it is of fundamental interest to establish protocols that allow the differentiation of ES cells into specific cell types. In recent years, several such differentiation procedures have been described for mouse and human embryonic stem cells. This unit describes a simple procedure that promotes the neuronal differentiation of mouse embryonic stem cells and yields a high proportion of midbrain dopaminergic neurons. Furthermore, this procedure permits the isolation of neural stem cell lines from mouse ES cells.

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Language(s): eng - English
 Dates: 2009-04-01
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: eDoc: 430326
DOI: 10.1002/0471142301.ns0306s47
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Title: Current Protocols in Neuroscience
Source Genre: Journal
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Pages: 21 Volume / Issue: 47 (Unit 3.6) Sequence Number: - Start / End Page: 3.6.1 - 3.6.21 Identifier: -