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  Application of an Epac Activator Enhances Neurotransmitter Release at Excitatory Central Synapses

Gekel, I., & Neher, E. (2008). Application of an Epac Activator Enhances Neurotransmitter Release at Excitatory Central Synapses. The Journal of Neuroscience, 28, 7991-8002. doi:10.1523/JNEUROSCI.0268-08.2008.

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 Urheber:
Gekel, I.1, Autor           
Neher, E.1, Autor           
Affiliations:
1Department of Membrane Biophysics, MPI for biophysical chemistry, Max Planck Society, ou_578579              

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 Zusammenfassung: cAMP regulates secretory processes through both PKA-independent and PKA-dependent signaling pathways. Their relative contributions to fast neurotransmission are unclear at present, although forskolin, which is generally believed to enhance intracellular cAMP levels by stimulation of adenylyl cyclase activity, was shown to increase vesicular release probability (p) and the number of releasable vesicles (N) in various neuronal preparations. Using low-frequency (0.2 Hz) electrophysiological recordings in the presence of the Epac-selective cAMP analog 8-pCPT-2′-O-Me-cAMP (ESCA1), we find that Epac activation by this analog accounts on average for 38% of the forskolin-induced increase in evoked EPSC amplitudes and for 100% of the forskolin-induced increase in miniature EPSC (mEPSC) frequency in dissociated autaptic neuronal cultures from mouse hippocampus. From paired-pulse facilitation experiments, and considering the enhancement of mEPSC frequency, we conclude that ESCA1-induced Epac activity is presynaptic in origin and increases p. In addition, preapplication of ESCA1 augmented a subsequent enhancement of evoked EPSC amplitudes by phorbol ester (PDBu). This effect was maximal when ESCA1 application preceded the PDBu application by 3 min. Because the PDBu response was abolished after downregulation of intracellular PKC activity, we conclude that ESCA1-induced Epac activation leads to presynaptic changes involving Epac-to-PKC signaling.

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Sprache(n): eng - English
 Datum: 2008-08
 Publikationsstatus: Erschienen
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 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: DOI: 10.1523/JNEUROSCI.0268-08.2008
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Titel: The Journal of Neuroscience
  Andere : The Journal of Neuroscience: the Official Journal of the Society for Neuroscience
  Kurztitel : J. Neurosci.
Genre der Quelle: Zeitschrift
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Ort, Verlag, Ausgabe: Washington, DC : Society of Neuroscience
Seiten: - Band / Heft: 28 Artikelnummer: - Start- / Endseite: 7991 - 8002 Identifikator: ISSN: 0270-6474
CoNE: https://pure.mpg.de/cone/journals/resource/954925502187_1