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  Superresolution by localization of quantum dots using blinking statistics

Lidke, K. A., Rieger, B., Jovin, T. M., & Heintzmann, R. (2005). Superresolution by localization of quantum dots using blinking statistics. Optics Express, 13(18), 7052-7062. Retrieved from http://www.opticsinfobase.org/oe/viewmedia.cfm?uri=oe-13-18-7052&seq=0.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-E823-4 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-D8A0-E
Genre: Journal Article

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 Creators:
Lidke, K. A.1, Author              
Rieger, B.1, Author              
Jovin, T. M.1, Author              
Heintzmann, R.1, Author              
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1Department of Molecular Biology, MPI for biophysical chemistry, Max Planck Society, ou_578628              

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Free keywords: Superresolution; Fluorescence microscopy; Image reconstruction–restoration; Pattern Recognition; Resolution
 Abstract: In microscopy, single fluorescence point sources can be localized with a precision several times greater than the resolution limit of the microscope. We show that the intermittent fluorescence or ’blinking’ of quantum dots can analyzed by an Independent Component Analysis so as to identify the light emitted by each individual nanoparticle, localize it precisely, and thereby resolve groups of closely spaced (< λ /30) quantum dots. Both simulated and experimental data demonstrate that this technique is superior to localization based on Maximum Likelihood Estimation under the assumption of point emitters. This technique has general application to any emitter with non-Gaussian temporal intensity distribution, including triplet state blinking. When applied to the labeling of structures, a high resolution ”image” consisting of individually localized points may be reconstructed leading to the term ”Pointillism”.

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Language(s): eng - English
 Dates: 2005-09-072005-09-05
 Publication Status: Published in print
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 Rev. Method: Peer
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Title: Optics Express
Source Genre: Journal
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Pages: - Volume / Issue: 13 (18) Sequence Number: - Start / End Page: 7052 - 7062 Identifier: -