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  The role of photon statistics in fluorescence anisotropy imaging

Lidke, K. A., Rieger, B., Jovin, T. M., & Heintzmann, R. (2005). The role of photon statistics in fluorescence anisotropy imaging. IEEE Transactions on Image Processing, 14(9), 1237-1245. Retrieved from http://ieeexplore.ieee.org/stamp/stamp.jsp?arnumber=1495497&isnumber=32133.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-E82C-1 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-D8C5-B
Genre: Journal Article

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 Creators:
Lidke, K. A.1, Author              
Rieger, B.1, Author              
Jovin, T. M.1, Author              
Heintzmann, R.1, Author              
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1Department of Molecular Biology, MPI for biophysical chemistry, Max Planck Society, ou_578628              

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Free keywords: Confocal microscopy; energy migration fluorescence resonance energy transfer; emFRET; enhanced green fluorescent protein (eGFP); scale selective filtering
 Abstract: Anisotropy imaging can be used to image resonance energy transfer between pairs of identical fluorophores and, thus, constitutes a powerful tool for monitoring protein homo-association in living single cells. The requirement for only a single fluorophore significantly simplifies biological preparation and interpretation. We use quantitative methods for the acquisition and image processing of anisotropy data that return the expected error of the anisotropy per pixel based on photon statistics. The analysis methods include calibration procedures and allow for a balance in spatial, anisotropy, and temporal resolution. They are featured here with anisotropy images of fluorescent calibration beads and enhanced green fluorescent protein complexes in live cells.

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Language(s): eng - English
 Dates: 2005-09-132005-09
 Publication Status: Published in print
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 Rev. Method: Peer
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Title: IEEE Transactions on Image Processing
Source Genre: Journal
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Pages: - Volume / Issue: 14 (9) Sequence Number: - Start / End Page: 1237 - 1245 Identifier: -