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  Reaching out for signals: Filopodia sense EGF and respond by directed retrograde transport of activated receptors

Lidke, D. S., Lidke, K. A., Rieger, B., Jovin, T. M., & Arndt-Jovin, D. J. (2005). Reaching out for signals: Filopodia sense EGF and respond by directed retrograde transport of activated receptors. Journal of Cell Biology, 170(4), 619-626. Retrieved from http://www.jcb.org/cgi/content/full/170/4/619.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-E85D-6 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-D718-6
Genre: Journal Article

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 Creators:
Lidke, D. S.1, Author              
Lidke, K. A.1, Author              
Rieger, B.1, Author              
Jovin, T. M.1, Author              
Arndt-Jovin, D. J.1, Author              
Affiliations:
1Department of Molecular Biology, MPI for biophysical chemistry, Max Planck Society, ou_578628              

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Free keywords: epidermal growth factor; EGF; epidermal growth factor receptor; EGFR; erbB1; retrograde transport; quantum dots
 Abstract: ErbB1 receptors situated on cellular filopodia undergo systematic retrograde transport after binding of the epidermal growth factor (EGF) and activation of the receptor tyrosine kinase. Specific inhibitors of the erbB1 receptor tyrosine kinase as well as cytochalasin D, a disruptor of the actin cytoskeleton, abolish transport but not free diffusion of the receptor–ligand complex. Diffusion constants and transport rates were determined with single molecule sensitivity by tracking receptors labeled with EGF conjugated to fluorescent quantum dots. Retrograde transport precedes receptor endocytosis, which occurs at the base of the filopodia. Initiation of transport requires the interaction and concerted activation of at least two liganded receptors and proceeds at a constant rate mediated by association with actin. These findings suggest a mechanism by which filopodia detect the presence and concentration of effector molecules far from the cell body and mediate cellular responses via directed transport of activated receptors.

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Language(s): eng - English
 Dates: 2005-08-162005-08-15
 Publication Status: Published in print
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 Rev. Method: Peer
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Title: Journal of Cell Biology
Source Genre: Journal
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Pages: - Volume / Issue: 170 (4) Sequence Number: - Start / End Page: 619 - 626 Identifier: -