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  Distinct kinetic changes in neurotransmitter release after SNARE protein cleavage

Sakaba, T., Stein, A., Jahn, R., & Neher, E. (2005). Distinct kinetic changes in neurotransmitter release after SNARE protein cleavage. Science, 309(5733), 491-494. doi:10.1126/science.1112645.

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Sakaba, T.1, Autor           
Stein, A.2, Autor           
Jahn, R.3, Autor           
Neher, E.4, Autor           
Affiliations:
1Research Group of Biophysics of Synaptic Transmission, MPI for biophysical chemistry, Max Planck Society, ou_578583              
2Research Group of Membrane Protein Biochemistry, MPI for Biophysical Chemistry, Max Planck Society, ou_2149675              
3Department of Neurobiology, MPI for biophysical chemistry, Max Planck Society, ou_578595              
4Department of Membrane Biophysics, MPI for biophysical chemistry, Max Planck Society, ou_578579              

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 Zusammenfassung: Neurotransmitter release is triggered by calcium ions and depends critically on the correct function of three types of SNARE [soluble N-ethylmaleimide–sensitive factor attachment protein (SNAP) receptor] proteins. With use of the large calyx of Held presynaptic terminal from rats, we found that cleavage of different SNARE proteins by clostridial neurotoxins caused distinct kinetic changes in neurotransmitter release. When elevating calcium ion concentration directly at the presynaptic terminal with the use of caged calcium, cleavage of SNAP-25 by botulinum toxin A (BoNT/A) produced a strong reduction in the calcium sensitivity for release, whereas cleavage of syntaxin using BoNT/C1 and synaptobrevin using tetanus toxin (TeNT) produced an all-or-nothing block without changing the kinetics of remaining vesicles. When stimulating release by calcium influx through channels, a difference between BoNT/C1 and TeNT emerged, which suggests that cleavage of synaptobrevin modifies the coupling between channels and release-competent vesicles.

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Sprache(n): eng - English
 Datum: 2005-07-15
 Publikationsstatus: Erschienen
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 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: DOI: 10.1126/science.1112645
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Titel: Science
  Kurztitel : Science
Genre der Quelle: Zeitschrift
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Ort, Verlag, Ausgabe: Washington, D.C. : American Association for the Advancement of Science
Seiten: - Band / Heft: 309 (5733) Artikelnummer: - Start- / Endseite: 491 - 494 Identifikator: ISSN: 0036-8075
CoNE: https://pure.mpg.de/cone/journals/resource/991042748276600_1