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  Imaging quantum dots switched on and off by photochromic Fluorescence Resonance Energy Transfer (pcFRET)

Jares-Erijman, E. A., Giordano, L., Spagnuolo, C., Lidke, K. A., & Jovin, T. M. (2005). Imaging quantum dots switched on and off by photochromic Fluorescence Resonance Energy Transfer (pcFRET). Molecular Crystals and Liquid Crystals, 430: DOI: 10.1080/15421400590946479, pp. 257-265. Retrieved from http://www.informaworld.com/smpp/ftinterface~content=a713989262~fulltext=713240928.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-E8F6-A Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-D9B8-1
Genre: Journal Article

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 Creators:
Jares-Erijman, E. A.1, Author              
Giordano, L., Author
Spagnuolo, C.1, Author              
Lidke, K. A.1, Author              
Jovin, T. M.1, Author              
Affiliations:
1Department of Molecular Biology, MPI for biophysical chemistry, Max Planck Society, ou_578628              

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Free keywords: diheteroarylethene; photochromism; quantum dot; FRET; pcFRET
 Abstract: The reversible modulation of the emission of CdSe=ZnS semiconductor nanocrystals (quantum dots) was achieved by binding photochromic diheteroarylethenesand switchable acceptors for fluorescence resonance energy transfer. A biotinylated diheteroarylethene derivative was bound to quantum dots bearing conjugated streptavidin, leading to an intensity decrease as a consequence of energy transfer to the closed form of the acceptor. Interconversion between the open and closed forms by irradiation with 365 and 546nm light enabled deactivation and activation, respectively, of the FRET process with a corresponding modulation of quantum dot emission, observed both in solution and by sequential wide-field imaging.

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 Dates: 2005-06-212005-06
 Publication Status: Published in print
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Title: Molecular Crystals and Liquid Crystals
Source Genre: Journal
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Pages: - Volume / Issue: 430 Sequence Number: DOI: 10.1080/15421400590946479 Start / End Page: 257 - 265 Identifier: -