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  Functional analysis of seven genes encoding eight translation initiation factor 4E (eIF4E) isoforms in Drosophila

Hernandez, G., Altmann, M., Sierra, J. M., Urlaub, H., Diez del Corral, R., Schwartz, P., et al. (2005). Functional analysis of seven genes encoding eight translation initiation factor 4E (eIF4E) isoforms in Drosophila. Mechanisms of Development, 122: doi:10.1016/j.mod.2004.11.011, pp. 529-543. Retrieved from http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6T9H-4F00RD2-1-C&_cdi=5115&_user=38661&_orig=browse&_coverDate=04%2F30%2F2005&_sk=998779995&view=c&wchp=dGLzVlz-zSkzV&md5=cdb88a9698452461508ca0f6f143a28b&ie=/sdarticle.pdf.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-E99B-1 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-0EF7-1
Genre: Journal Article

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 Creators:
Hernandez, G.1, Author              
Altmann, M., Author
Sierra, J. M., Author
Urlaub, H.2, Author              
Diez del Corral, R., Author
Schwartz, P., Author
Rivera-Pomar, R.3, Author              
Affiliations:
1Department of Molecular Biology, MPI for biophysical chemistry, Max Planck Society, ou_578628              
2Research Group of Bioanalytical Mass Spectrometry, MPI for biophysical chemistry, Max Planck Society, ou_578613              
3Department of Cellular Biochemistry, MPI for biophysical chemistry, Max Planck Society, ou_578576              

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Free keywords: initiation of translation; eIF4E; eIF4E isoforms; drosophila; l(3)67Af
 Abstract: The Drosophila genome-sequencing project has revealed a total of seven genes encoding eight eukaryotic initiation factor 4E (eIF4E) isoforms. Four of them (eIF4E-1,2, eIF4E-3, eIF4E-4 and eIF4E-5) share exon/intron structure in their carboxy-terminal part and form a cluster in the genome. All eIF4E isoforms bind to the cap (m⁷GpppN) structure. All of them, except eIF4E-6 and eIF4E-8 were able to interact with Drosophila eIF4G or eIF4E-binding protein (4E-BP). eIF4E-1, eIF4E-2, eIF4E-3, eIF4E-4 and eIF4E-7 rescued a yeast eIF4Edeficient mutant in vivo. Only eIF4E-1 mRNAs and, at a significantly lower level, eIF4E3 and eIF4E-8 are expressed in embryos and throughout the life cycle of the fly. The transcripts of the remaining isoforms were detected from the third instar larvae onwards. This indicates the cap-binding activity relies mostly on eIF4E-1 during embryogenesis. This agrees with the proteomic analysis of the eIF4F complex purified from embryos and with the rescue of l(3)67Af, an embryonic lethal mutant for the eIF4E-1,2 gene, by transgenic expression of eIF4E-1. Overexpression of eIF4E-1 in wild-type embryos and eye imaginal discs results in phenotypic defects in a dose-dependent manner.

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 Dates: 2005-04-052005-04
 Publication Status: Published in print
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Title: Mechanisms of Development
Source Genre: Journal
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Pages: - Volume / Issue: 122 Sequence Number: doi:10.1016/j.mod.2004.11.011 Start / End Page: 529 - 543 Identifier: -