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  Gain-of-Function Screen for Genes That Affect Drosophila Muscle Pattern Formation

Staudt, N., Molitor, A., Somogyi, K., Mata, J., Curado, S., Eulenberg, K. G., et al. (2005). Gain-of-Function Screen for Genes That Affect Drosophila Muscle Pattern Formation. PLoS Genetics, 1(4): e55. doi:10.1371/journal.pgen.0010055.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-EAD0-E Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0029-281F-E
Genre: Journal Article

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PLoS_Genet_1(4)_e55.pdf (Publisher version), 2MB
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Staudt, N.1, Author              
Molitor, A.1, Author              
Somogyi, K., Author
Mata, J., Author
Curado, S., Author
Eulenberg, K. G., Author
Meise, M., Author
Siegmund, T., Author
Haeder, T.1, Author              
Hilfiker, A., Author
Broenner, G.1, Author              
Ephrussi, A., Author
Rroth, P., Author
Cohen, S. M., Author
Fellert, S.1, Author              
Chung, H. R.1, Author              
Piepenburg, O.1, Author              
Schaefer, U.1, Author              
Jäckle, H.1, Author              
Vorbrüggen, G.2, Author              
Affiliations:
1Department of Molecular Developmental Biology, MPI for biophysical chemistry, Max Planck Society, ou_578590              
2Research Group of Molecular Cell Dynamics, MPI for biophysical chemistry, Max Planck Society, ou_578593              

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 Abstract: This article reports the production of an EP-element insertion library with more than 3,700 unique target sites within the Drosophila melanogaster genome and its use to systematically identify genes that affect embryonic muscle pattern formation. We designed a UAS/GAL4 system to drive GAL4-responsive expression of the EP-targeted genes in developing apodeme cells to which migrating myotubes finally attach and in an intrasegmental pattern of cells that serve myotubes as a migration substrate on their way towards the apodemes. The results suggest that misexpression of more than 1.5% of the Drosophila genes can interfere with proper myotube guidance and/or muscle attachment. In addition to factors already known to participate in these processes, we identified a number of enzymes that participate in the synthesis or modification of protein carbohydrate side chains and in Ubiquitin modifications and/or the Ubiquitin-dependent degradation of proteins, suggesting that these processes are relevant for muscle pattern formation.

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Language(s): eng - English
 Dates: 2005
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: eDoc: 256118
DOI: 10.1371/journal.pgen.0010055
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Title: PLoS Genetics
Source Genre: Journal
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Pages: - Volume / Issue: 1 (4) Sequence Number: e55 Start / End Page: - Identifier: -