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  Ensemble and single particle photophysical properties (Two-photon excitation, anisotropy, FRET, lifetime, spectral conversion) of commercial quantum dots in solution and in live cells

Grecco, H. E., Lidke, K. A., Heintzmann, R., Lidke, D. S., Spagnuolo, C., Martinez, O. E., et al. (2004). Ensemble and single particle photophysical properties (Two-photon excitation, anisotropy, FRET, lifetime, spectral conversion) of commercial quantum dots in solution and in live cells. Miscroscopy Research and Technique, 65: DOI 10.1002/jemt.20129, pp. 169-179. Retrieved from http://www3.interscience.wiley.com/cgi-bin/fulltext/109861786/PDFSTART.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-EB6E-8 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-D816-8
Genre: Journal Article

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206547.pdf (Publisher version), 0B
 
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 Creators:
Grecco, H. E., Author
Lidke, K. A.1, Author              
Heintzmann, R.1, Author              
Lidke, D. S.1, Author              
Spagnuolo, C.1, Author              
Martinez, O. E., Author
Jares-Erijman, E. A.1, Author              
Jovin, T. M.1, Author              
Affiliations:
1Department of Molecular Biology, MPI for biophysical chemistry, Max Planck Society, ou_578628              

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Free keywords: nanoparticles; fluorescence microscopy; growth factor receptors
 Abstract: In this work we characterized streptavidin conjugated quantum dots (QDs) manufactured by Quantum Dot Corporation. We present data on: (i) two-photon excitation; (ii) fluorescence lifetimes; (iii) ensemble and single QD emission anisotropy; (iv) QDs as donors for Förster resonance energy transfer (FRET); and (v) spectral conversion of QDs exposed to high intensity illumination. We also demonstrate the utility of QDs for (i) imaging the binding and uptake of biotinylated transferrin on living cells, and (ii) resolving by fluorescence lifetime imaging microscopy (FLIM) signals originating from QDs from those of spatially and spectrally overlapping visible fluorescent proteins (VFPs).

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Language(s): eng - English
 Dates: 2005-01-032004-12-22
 Publication Status: Published in print
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Title: Miscroscopy Research and Technique
Source Genre: Journal
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Pages: - Volume / Issue: 65 Sequence Number: DOI 10.1002/jemt.20129 Start / End Page: 169 - 179 Identifier: -