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  Scaffolding by ERK3 regulates MK5 in development.

Schumacher, S., Laass, K., Kant, S., Shi, Y., Visel, A., Gruber, A. D., et al. (2004). Scaffolding by ERK3 regulates MK5 in development. EMBO Journal, 23(24), 4770-4779. Retrieved from http://www.nature.com/emboj/journal/v23/n24/full/7600467a.html.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-EB86-E Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-0957-4
Genre: Journal Article

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 Creators:
Schumacher, S., Author
Laass, K., Author
Kant, S., Author
Shi, Y., Author
Visel, A.1, Author              
Gruber, A. D., Author
Kotlyarov, A., Author
Gaestel, M., Author
Affiliations:
1Department of Genes and Behavior, MPI for biophysical chemistry, Max Planck Society, ou_persistent34              

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Free keywords: Animals; Cell Line; Embryo/anatomy & histology/physiology; Embryonic Development/physiology; Enzyme Activation; Humans; In Situ Hybridization; Mice; Mice, Knockout; Mitogen-Activated Protein Kinase 6/genetics/metabolism; Protein Structure, Tertiary; Protein Transport/physiology; Protein-Serine-Threonine Kinases/genetics/metabolism; Recombinant Fusion Proteins/genetics/metabolism; Research Support, Non-U.S. Gov't; Signal Transduction/physiology; Two-Hybrid System Techniques
 Abstract: Extracellular-regulated kinase 3 (ERK3, MAPK6) is an atypical member of the ERKs, lacking the threonine and tyrosine residues in the activation loop, carrying a unique C-terminal extension and being mainly regulated by its own protein stability and/or by autophosphorylation. Here we show that ERK3 specifically interacts with the MAPK-activated protein kinase 5 (MK5 or PRAK) in vitro and in vivo. Expression of ERK3 in mammalian cells leads to nuclear-cytoplasmic translocation and activation of MK5 and to phosphorylation of both ERK3 and MK5. Remarkably, activation of MK5 is independent of ERK3 enzymatic activity, but depends on its own catalytic activity as well as on a region in the C-terminal extension of ERK3. In mouse embryonic development, mRNA expression patterns of ERK3 and MK5 suggest spatiotemporal coexpression of both kinases. Deletion of MK5 leads to strong reduction of ERK3 protein levels and embryonic lethality at about stage E11, where ERK3 expression in wild-type mice is maximum, indicating a role of this signalling module in development.

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Language(s): eng - English
 Dates: 2004-12-08
 Publication Status: Published in print
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 Rev. Method: Peer
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Title: EMBO Journal
Source Genre: Journal
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Pages: - Volume / Issue: 23 (24) Sequence Number: - Start / End Page: 4770 - 4779 Identifier: -