English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Diffusion of microspheres in sealed and open microarrays

Rieger, B., Dietrich, H. R. C., van den Doel, R. L., & van Vliet, L. J. (2004). Diffusion of microspheres in sealed and open microarrays. Microscopy Research and Technique, 65: DOI 10.1002/jemt.20128, pp. 218-225. Retrieved from http://www3.interscience.wiley.com/cgi-bin/fulltext/109861802/PDFSTART.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-EB92-1 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-D912-8
Genre: Journal Article

Files

show Files
hide Files
:
206709.pdf (Publisher version), 0B
 
File Permalink:
-
Name:
206709.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Biophysical Chemistry (Karl Friedrich Bonhoeffer Institute), Göttingen; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Rieger, B.1, Author              
Dietrich, H. R. C., Author
van den Doel, R. L., Author
van Vliet, L. J., Author
Affiliations:
1Department of Molecular Biology, MPI for biophysical chemistry, Max Planck Society, ou_578628              

Content

show
hide
Free keywords: Brownian motion; diffusion; mean square displacement; micro arrays; microarrays; micro beads
 Abstract: In several experiments, we study the diffusion of microspheres with different radii in microarrays filled with a variety of aqueous solutions of ethylene glycol. We study diffusion in open and closed (sealed) microarrays. In sealed nanoliter wells, the tracers show pure diffusion, whereas in open reactors, a radial outward-directed evaporation-induced liquid flow is superimposed onto the diffusion. In general, one of the following quantities can be calculated if the others are known: the temperature, the viscosity of the medium, the radius of the microbeads, or the diffusion constant. The estimated diffusion constants in closed microarrays are in good agreement with theoretical predictions based on the Brownian motion. We monitor the motion of the microbeads under a microscope and extract their paths in time from the digital recordings. Ambiguous paths due to the crossing of two trajectories can be detected. We show that low microsphere concentrations or high viscosities do not hamper a robust estimation of the diffusion parameters.

Details

show
hide
Language(s): eng - English
 Dates: 2005-01-102004-12
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Microscopy Research and Technique
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 65 Sequence Number: DOI 10.1002/jemt.20128 Start / End Page: 218 - 225 Identifier: -