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  A unique PDZ ligand in PKCalpha confers induction of cerebellar long-term synaptic depression.

Leitges, M., Kovac, J., Plomann, M., & Linden, D. J. (2004). A unique PDZ ligand in PKCalpha confers induction of cerebellar long-term synaptic depression. Neuron, 44(4), 585-594. Retrieved from http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15541307.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-EBC7-B Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0029-D671-6
Genre: Journal Article

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Leitges, M.1, Author              
Kovac, J.2, Author              
Plomann, M., Author
Linden, D. J., Author
Affiliations:
1Department of Genes and Behavior, MPI for biophysical chemistry, Max Planck Society, ou_persistent34              
2Research Group on Circadian Rythms, MPI for biophysical chemistry, Max Planck Society, ou_578594              

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Free keywords: 3T3 Cells; Animals; Humans; Isoenzymes/genetics/metabolism; Ligands; Long-Term Depression (Physiology)/physiology; Mice; Patch-Clamp Techniques; Protein Kinase C/genetics/metabolism Protein-Serine-Threonine Kinases/metabolism; Purkinje Cells/enzymology; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.; Synapses/physiology; Transfection
 Abstract: Induction of cerebellar long-term depression (LTD) requires a postsynaptic cascade involving activation of mGluR1 and protein kinase C (PKC). Our understanding of this process has been limited by the fact that PKC is a large family of molecules, many isoforms of which are expressed in the relevant postsynaptic compartment, the cerebellar Purkinje cell. Here, we report that LTD is absent in Purkinje cells in which the alpha isoform of PKC has been reduced by targeted RNA interference or in cells derived from PKCalpha null mice. In both of these cases, LTD could be rescued by expression of PKCalpha but not other PKC isoforms. The special role of PKCalpha in cerebellar LTD is likely to derive from its unique PDZ ligand (QSAV). When this motif is mutated, PKCalpha no longer supports LTD. Conversely, when this PDZ ligand is inserted in a nonpermissive isoform, PKCgamma, it confers the capacity for LTD induction.

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Language(s): eng - English
 Dates: 2004-11-18
 Publication Status: Published in print
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Title: Neuron
Source Genre: Journal
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Pages: - Volume / Issue: 44 (4) Sequence Number: - Start / End Page: 585 - 594 Identifier: -