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  Evidence for a role of protein kinase C-alpha in urine concentration.

Yao, L., Huang, D. Y., Pfaff, I. L., Nie, X., Leitges, M., & Vallon, V. (2004). Evidence for a role of protein kinase C-alpha in urine concentration. American Journal of Physiology, 287(2), F299-F304. Retrieved from http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15039142.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-ECA0-A Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-EE62-B
Genre: Journal Article

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 Creators:
Yao, L., Author
Huang, D. Y., Author
Pfaff, I. L., Author
Nie, X., Author
Leitges, M.1, Author              
Vallon, V., Author
Affiliations:
1Department of Genes and Behavior, MPI for biophysical chemistry, Max Planck Society, ou_persistent34              

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Free keywords: Animals; Aquaporins/metabolism; Diffusion Chambers, Culture; Glomerular Filtration Rate; Kidney/anatomy & histology/physiology; Kidney Concentrating Ability/physiology; Kidney Medulla/metabolism; Male; Mice; Mice, Knockout; Morpholines/pharmacology; Natriuresis; Organ Size; Protein Kinase C/physiology; Receptors, Vasopressin/antagonists & inhibitors; Research Support, Non-U.S. Gov't; Sodium/deficiency; Sodium, Dietary/administration & dosage; Spiro Compounds/pharmacology; Water/pharmacology; Water Deprivation/physiology
 Abstract: In mouse kidney, the conventional protein kinase C (PKC) isoenzyme alpha is expressed in glomeruli, the cortical collecting duct (intercalated cells only), and medullary collecting duct. To get insights on its function, PKC-alpha knockout (-/-) and wild-type (+/+) mice were studied. When provided free access to water, PKC-alpha -/- mice showed approximately 50% greater urine flow rate and lower urinary osmolality in 24-h metabolic cage experiments despite a greater urinary vasopressin-to-creatinine ratio vs. PKC-alpha +/+ mice. Renal albumin excretion was not different. Clearance experiments under inactin/ketamine anesthesia revealed a modestly reduced glomerular filtration rate and showed a reduced absolute and fractional renal fluid reabsorption in PKC-alpha -/- mice. The sodium-restricting response to a low-sodium diet was unaffected in PKC-alpha -/- mice. Urinary osmolality was reduced to similar hypotonic levels in PKC-alpha -/- and +/+ mice during acute oral water loading or application of the vasopressin V(2)-receptor antagonist SR-121463. In comparison, the lower urinary osmolality observed in PKC-alpha -/- mice vs. wild-type mice under basal conditions persisted during water restriction for 36 h. In conclusion, PKC-alpha appears not to play a major role in renal sodium reabsorption but, consistent with its expression in the medullary collecting duct, contributes to urinary concentration in mice. Considering that PKC-beta I and -beta II are coexpressed with PKC-alpha in mouse medullary collecting duct, the present results indicate that conventional PKC isoenzymes cannot fully compensate for each other.

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Language(s): eng - English
 Dates: 2004-08
 Publication Status: Published in print
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 Rev. Method: Peer
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Title: American Journal of Physiology
Source Genre: Journal
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Pages: - Volume / Issue: 287 (2) Sequence Number: - Start / End Page: F299 - F304 Identifier: -