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  Formation of DNA nanoparticles in the presence of novel polyamine analogues: a laser light scattering and atomic force microscopic study

Vijayanathan, V., Thomas, T., Antony, T., Shirahata, A., & Thomas, T. J. (2004). Formation of DNA nanoparticles in the presence of novel polyamine analogues: a laser light scattering and atomic force microscopic study. Nucleic Acids Research, 32(1): DOI: 10.1093/nar/gkg936, pp. 127-134. Retrieved from http://nar.oxfordjournals.org/cgi/content/full/32/1/127.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-ED23-D Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-DAD2-0
Genre: Journal Article

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 Creators:
Vijayanathan, V., Author
Thomas, T.1, Author              
Antony, T.2, Author              
Shirahata, A., Author
Thomas, T. J., Author
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1Department of Molecular Cell Biology, MPI for biophysical chemistry, Max Planck Society, ou_578585              
2Department of Molecular Biology, MPI for biophysical chemistry, Max Planck Society, ou_578628              

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 Abstract: We synthesized a pentamine (3-3-3-3) and two hexamine (3-3-3-3-3 and 3-4-3-4-3) analogues of the natural polyamine, spermine (3-4-3) and studied their effectiveness in condensing pGL3 plasmid DNA, using light scattering and atomic force microscopic (AFM) techniques. The midpoint concentration of the polyamines on pGL3 condensation (EC50) was 11.3, 10.6, 1.5, 0.49 and 0.52 mM, respectively, for 3-4-3, norspermine (3-3-3), 3-3-3-3, 3-3-3-3-3 and 3-4-3-4-3 in 10 mM Na cacodylate buffer. Dynamic laser light scattering study showed a decrease in hydrodynamic radii of plasmid DNA particles as the number of positive charges on the polyamines increased. AFM data showed the presence of toroids with outer diameter of 117-191 nm for different polyamines, and a mean height of 2.61 ± 0.77 nm. AFM results also revealed the presence of intermediate structures, including those showing circumferential winding of DNA to toroids. The dependence of the EC₅₀ on Na⁺ concentration suggests different modes of binding of spermine and its higher valent analogues with DNA. Our results show a 20-fold increase in the efficacy of hexamines for DNA condensation compared to spermine, and provide new insights into the mechanism(s) of DNA nanoparticle formation. These studies might help to develop novel nonviral gene delivery vehicles.

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Language(s): eng - English
 Dates: 2005-04-122004-06
 Publication Status: Published in print
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 Rev. Method: Peer
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Title: Nucleic Acids Research
Source Genre: Journal
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Pages: - Volume / Issue: 32 (1) Sequence Number: DOI: 10.1093/nar/gkg936 Start / End Page: 127 - 134 Identifier: -