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  FRET Imaging

Jares-Erijman, E. A., & Jovin, T. M. (2003). FRET Imaging. Nature biotechnology, 21(11), 1387-1395. Retrieved from http://www.nature.com/nbt/journal/v21/n11/pdf/nbt896.pdf.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-EFE0-8 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-E3A2-4
Genre: Journal Article

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Jares-Erijman, E. A.1, Author              
Jovin, T. M.1, Author              
Affiliations:
1Department of Molecular Biology, MPI for biophysical chemistry, Max Planck Society, ou_578628              

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 Abstract: Förster (or Fluorescence) Resonance Energy Transfer (FRET) generates fluorescence signals sensitive to molecular conformation, association, and separation in the 1-10 nm range. We introduce a new photophysical framework for the phenomenon and apply it to the wide range of FRET techniques adapted to imaging systems. A systematic catalogue of 21 such methods is presented, including several new approaches proposed as suitable prospects for implementation. We conclude with a brief discussion of selected probe issues and anticipated future developments extending from single molecule to live cell applications.

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Language(s): eng - English
 Dates: 2003-10-31
 Publication Status: Published in print
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 Rev. Method: Peer
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Title: Nature biotechnology
Source Genre: Journal
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Pages: - Volume / Issue: 21 (11) Sequence Number: - Start / End Page: 1387 - 1395 Identifier: -