English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Class IHLA oligomerization at the surface of B cells is controlled by exogenous β₂-microglobulin: implications in activation of cytotoxic T lymphocytes

Bodnar, A., Bacso, Z., Jenei, A., Jovin, T. M., Edidin, M., Damjanovich, S., et al. (2003). Class IHLA oligomerization at the surface of B cells is controlled by exogenous β₂-microglobulin: implications in activation of cytotoxic T lymphocytes. International Immunology, 15(3), 331-339. Retrieved from http://intl-intimm.oxfordjournals.org/cgi/reprint/15/3/331.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-F155-2 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-E3E3-0
Genre: Journal Article

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Bodnar, A., Author
Bacso, Z., Author
Jenei, A., Author
Jovin, T. M.1, Author              
Edidin, M., Author
Damjanovich, S., Author
Matko, J., Author
Affiliations:
1Department of Molecular Biology, MPI for biophysical chemistry, Max Planck Society, ou_578628              

Content

show
hide
Free keywords: β₂-microglobulin; cell-surface HLA cluster; cytotoxicity; fluorescence resonance energy transfer; free heavy chain; T cell activation
 Abstract: Submicroscopic molecular clusters (oligomers) of class I HLA have been detected by physical techniques [e.g. fluorescence resonance energy transfer (FRET) and single particle tracking of molecular diffusion] at the surface of various activated and transformed human cells, including B lymphocytes. Here, the sensitivity of this homotypic association to exogenous β₂-microglobulin (β₂m) and the role of free heavy chains (FHC) in class I HLA oligomerization were investigated on a B lymphoblastoid cell line, JY. Scanning near-field optical microscopy and FRET data both demonstrated that FHC and class I HLA heterodimers are co-clustered at the cell surface. Culturing the cells with excess β₂m resulted in a reduced co-clustering and decreased molecular homotypic association, as assessed by FRET. The decreased HLA clustering on JY target cells (antigen-presenting cells) was accompanied with their reduced susceptibility to specific lysis by allospecific CD8⁺ cytotoxic T lymphocytes (CTL). JY B cells with reduced HLA clustering also provoked significantly weaker T cell activation signals, such as lower expression of CD69 activation marker and lower magnitude of TCR down-regulation, than did the untreated B cells. These results together suggest that the actual level of β₂m available at the cell surface can control CTL activation and the subsequent cytotoxic effector function through regulation of the homotypic HLA-I association. This might be especially important in some inflammatory and autoimmune diseases where elevated serum β₂m levels are reported.

Details

show
hide
Language(s): eng - English
 Dates: 2004-07-272003-03
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: International Immunology
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 15 (3) Sequence Number: - Start / End Page: 331 - 339 Identifier: -