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  Sr2+ binding to the Ca2+ binding site of the synaptotagmin 1 C2B domain triggers fast exocytosis without stimulating SNARE interactions

Shin, O. H., Rhee, J. S., Tang, J., Sugita, S., Rosenmund, C., & Suedhof, T. C. (2003). Sr2+ binding to the Ca2+ binding site of the synaptotagmin 1 C2B domain triggers fast exocytosis without stimulating SNARE interactions. Neuron, 37(1), 99-108. Retrieved from http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6WSS-47P8K6X-F-1&_cdi=7054&_user=38661&_pii=S0896627302011455&_orig=search&_coverDate=01%2F09%2F2003&_sk=999629998&view=c&wchp=dGLzVlb-zSkzS&md5=a356a79b204d8e4c7fb3b62e5be6473a&ie=/sdarticle.pdf.

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Shin, O. H., Author
Rhee, J. S.1, Author           
Tang, J., Author
Sugita, S., Author
Rosenmund, C.1, Author           
Suedhof, T. C., Author
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1Department of Membrane Biophysics, MPI for biophysical chemistry, Max Planck Society, ou_578579              

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 Abstract: Sr2+ triggers neurotransmitter release similar to Ca2+, but less efficiently. We now show that in synaptotagmin 1 knockout mice, the fast component of both Ca2+- and Sr2+-induced release is selectively impaired, suggesting that both cations partly act by binding to synaptotagmin 1. Both the C(2)A and the C2B domain of synaptotagmin 1 bind Ca2+ in phospholipid complexes, but only the C2B domain forms Sr2+/phospholipid complexes; therefore, Sr2+ binding to the C2B domain is sufficient to trigger fast release, although with decreased efficacy. Ca2+ induces binding of the synaptotagmin C, domains to SNARE proteins, whereas Sr2+ even at high concentrations does not. Thus, triggering of the fast component of release by Sr2+ as a Ca2+ agonist involves the formation of synaptotagmin/ phospholipid complexes, but does not require stimulated SNARE binding.

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Language(s): eng - English
 Dates: 2003-01-09
 Publication Status: Issued
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 Rev. Type: Peer
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Title: Neuron
Source Genre: Journal
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Pages: - Volume / Issue: 37 (1) Sequence Number: - Start / End Page: 99 - 108 Identifier: -